Existence of lysogenic bacteriophages in Bacillus thuringiensis type strains.

Jong Yul Roh; Jong Bin Park; Qin Liu; Song Eun Kim; Xueying Tao; Tae Woong Choi; Jae Young Choi; Woo Jin Kim; Byung Rae Jin; Yeon Ho Je

doi:10.1016/j.jip.2013.04.008

Existence of lysogenic bacteriophages in Bacillus thuringiensis type strains.

Jong Yul Roh, Jong Bin Park, Qin Liu, Song Eun Kim, Xueying Tao, Tae Woong Choi, Jae Young Choi, Woo Jin Kim, Byung Rae Jin, Yeon Ho Je

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We screened the existence of bacteriophages in 67 Bacillus thuringiensis type strains by phage DNA extraction and PCR using phage terminase small subunit (TerS)-specific primers to the supernatants and the precipitated pellets of Bt cultures, and by transmission electron microscopy. The various bacteriophages were observed from the supernatants of 22 type strains. Ten type strains showed the extracted phage DNAs and the amplified fragment by TerS PCR but 12 type strains showed only the phage DNAs. Their morphological characteristic suggests that they belong to Family Siphoviridae which had a long tail and symmetrical head.

Original language	English
Pages (from-to)	228-231
Number of pages	4
Journal	Journal of Invertebrate Pathology
Volume	113
Issue number	3
DOIs	https://doi.org/10.1016/j.jip.2013.04.008
Publication status	Published - Jul 2013

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10.1016/j.jip.2013.04.008

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title = "Existence of lysogenic bacteriophages in Bacillus thuringiensis type strains.",

abstract = "We screened the existence of bacteriophages in 67 Bacillus thuringiensis type strains by phage DNA extraction and PCR using phage terminase small subunit (TerS)-specific primers to the supernatants and the precipitated pellets of Bt cultures, and by transmission electron microscopy. The various bacteriophages were observed from the supernatants of 22 type strains. Ten type strains showed the extracted phage DNAs and the amplified fragment by TerS PCR but 12 type strains showed only the phage DNAs. Their morphological characteristic suggests that they belong to Family Siphoviridae which had a long tail and symmetrical head.",

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AU - Roh, Jong Yul

AU - Park, Jong Bin

AU - Liu, Qin

AU - Kim, Song Eun

AU - Tao, Xueying

AU - Choi, Tae Woong

AU - Choi, Jae Young

AU - Kim, Woo Jin

AU - Jin, Byung Rae

AU - Je, Yeon Ho

PY - 2013/7

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AB - We screened the existence of bacteriophages in 67 Bacillus thuringiensis type strains by phage DNA extraction and PCR using phage terminase small subunit (TerS)-specific primers to the supernatants and the precipitated pellets of Bt cultures, and by transmission electron microscopy. The various bacteriophages were observed from the supernatants of 22 type strains. Ten type strains showed the extracted phage DNAs and the amplified fragment by TerS PCR but 12 type strains showed only the phage DNAs. Their morphological characteristic suggests that they belong to Family Siphoviridae which had a long tail and symmetrical head.

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EP - 231

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

IS - 3

ER -

Existence of lysogenic bacteriophages in Bacillus thuringiensis type strains.

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