Abstract
Recombinant endostatin was transiently expressed in Agrobacterium- inoculated leaf-disks of Nicotiana tabacum var. xanthi with a molecular size of 23 kDa. Expression of endostatin from a replicating vector based on tomato golden mosaic virus (TGMV) was 170% higher at the transcript level and double higher at the protein level than from a control vector of a non-replicating construct. Purified recombinant endostatin from tobacco leaf-disks has an anti-proliferative effect on bovine endothelial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 321-327 |
| Number of pages | 7 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 80 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2005 |
Bibliographical note
Funding Information:This work was supported by grants from the Biogreen 21 project and from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University.
Keywords
- Endostatin
- Leaf-disks
- Nicotiana tabacum
- Tomato golden mosaic virus
- Transient expression
Fingerprint
Dive into the research topics of 'Expression of recombinant endostatin in Agrobacterium-inoculated leaf-disks of Nicotiana tabacum var. xanthi'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver