Expression of secreted mucins (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound mucin (MUC4) in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae

Chung Hyun Kim; Yeonsu Oh; Kiwon Han; Hwi Won Seo; Duyeol Kim; Ikjae Kang; Changhoon Park; Ki Young Jang; Sung Hoon Kim; Chanhee Chae

doi:10.1016/j.rvsc.2011.04.016

Expression of secreted mucins (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound mucin (MUC4) in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae

Chung Hyun Kim
, Yeonsu Oh
, Kiwon Han
, Hwi Won Seo
, Duyeol Kim
, Ikjae Kang
, Changhoon Park
, Ki Young Jang
, Sung Hoon Kim
, Chanhee Chae

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The expression patterns of different secreted (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound (MUC4) mucins were determined immunohistochemically in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae. Forty-seven-week-old colostrum-deprived pigs were randomly allocated to infected (n=20) or control groups (n=20). Five infected and uninfected pigs were euthanized at 0, 6, 12, and 48. h post-inoculation (hpi). In the infected pigs, the expression of both types of mucins, which were invariably observed, was associated with bronchiolar and respiratory bronchiolar lesions. Strong positive mucin signals were seen on the surface of bronchiolar and respiratory bronchiolar epithelium with neutrophil infiltration. The mean mucin-positive area peaked at 6. hpi and decreased significantly to control levels by 48. hpi on the surface of the bronchiolar and respiratory bronchiolar epithelium. Further studies are needed to establish the functional relationship between mucin expression and the host defense mechanism against A. pleuropneumoniae in the lungs of infected pigs.

Original language	English
Pages (from-to)	486-491
Number of pages	6
Journal	Research in Veterinary Science
Volume	92
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2011.04.016
Publication status	Published - Jun 2012

Bibliographical note

Funding Information:
This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. This research was also supported by the Research Institute for Veterinary Science (RIVS) of the College of Veterinary Medicine, the Brain Korea 21 Program for Veterinary Science, and Korea Research Foundation Grant (KRF-2006-005-J02902).

Keywords

Actinobacillus pleuropneumoniae
Immunohistochemistry
Lung
Mucin
Pig

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10.1016/j.rvsc.2011.04.016

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Kim, C. H., Oh, Y., Han, K., Seo, H. W., Kim, D., Kang, I., Park, C., Jang, K. Y., Kim, S. H., & Chae, C. (2012). Expression of secreted mucins (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound mucin (MUC4) in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae. Research in Veterinary Science, 92(3), 486-491. https://doi.org/10.1016/j.rvsc.2011.04.016

@article{9669762021ac4ad185036f4781844fef,

title = "Expression of secreted mucins (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound mucin (MUC4) in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae",

abstract = "The expression patterns of different secreted (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound (MUC4) mucins were determined immunohistochemically in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae. Forty-seven-week-old colostrum-deprived pigs were randomly allocated to infected (n=20) or control groups (n=20). Five infected and uninfected pigs were euthanized at 0, 6, 12, and 48. h post-inoculation (hpi). In the infected pigs, the expression of both types of mucins, which were invariably observed, was associated with bronchiolar and respiratory bronchiolar lesions. Strong positive mucin signals were seen on the surface of bronchiolar and respiratory bronchiolar epithelium with neutrophil infiltration. The mean mucin-positive area peaked at 6. hpi and decreased significantly to control levels by 48. hpi on the surface of the bronchiolar and respiratory bronchiolar epithelium. Further studies are needed to establish the functional relationship between mucin expression and the host defense mechanism against A. pleuropneumoniae in the lungs of infected pigs.",

keywords = "Actinobacillus pleuropneumoniae, Immunohistochemistry, Lung, Mucin, Pig",

author = "Kim, \{Chung Hyun\} and Yeonsu Oh and Kiwon Han and Seo, \{Hwi Won\} and Duyeol Kim and Ikjae Kang and Changhoon Park and Jang, \{Ki Young\} and Kim, \{Sung Hoon\} and Chanhee Chae",

note = "Funding Information: This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. This research was also supported by the Research Institute for Veterinary Science (RIVS) of the College of Veterinary Medicine, the Brain Korea 21 Program for Veterinary Science, and Korea Research Foundation Grant (KRF-2006-005-J02902).",

year = "2012",

month = jun,

doi = "10.1016/j.rvsc.2011.04.016",

language = "English",

volume = "92",

pages = "486--491",

journal = "Research in Veterinary Science",

issn = "0034-5288",

publisher = "Elsevier B.V.",

number = "3",

}

Kim, CH, Oh, Y, Han, K, Seo, HW, Kim, D, Kang, I, Park, C, Jang, KY, Kim, SH & Chae, C 2012, 'Expression of secreted mucins (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound mucin (MUC4) in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae', Research in Veterinary Science, vol. 92, no. 3, pp. 486-491. https://doi.org/10.1016/j.rvsc.2011.04.016

TY - JOUR

T1 - Expression of secreted mucins (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound mucin (MUC4) in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae

AU - Kim, Chung Hyun

AU - Oh, Yeonsu

AU - Han, Kiwon

AU - Seo, Hwi Won

AU - Kim, Duyeol

AU - Kang, Ikjae

AU - Park, Changhoon

AU - Jang, Ki Young

AU - Kim, Sung Hoon

AU - Chae, Chanhee

N1 - Funding Information: This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. This research was also supported by the Research Institute for Veterinary Science (RIVS) of the College of Veterinary Medicine, the Brain Korea 21 Program for Veterinary Science, and Korea Research Foundation Grant (KRF-2006-005-J02902).

PY - 2012/6

Y1 - 2012/6

N2 - The expression patterns of different secreted (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound (MUC4) mucins were determined immunohistochemically in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae. Forty-seven-week-old colostrum-deprived pigs were randomly allocated to infected (n=20) or control groups (n=20). Five infected and uninfected pigs were euthanized at 0, 6, 12, and 48. h post-inoculation (hpi). In the infected pigs, the expression of both types of mucins, which were invariably observed, was associated with bronchiolar and respiratory bronchiolar lesions. Strong positive mucin signals were seen on the surface of bronchiolar and respiratory bronchiolar epithelium with neutrophil infiltration. The mean mucin-positive area peaked at 6. hpi and decreased significantly to control levels by 48. hpi on the surface of the bronchiolar and respiratory bronchiolar epithelium. Further studies are needed to establish the functional relationship between mucin expression and the host defense mechanism against A. pleuropneumoniae in the lungs of infected pigs.

AB - The expression patterns of different secreted (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound (MUC4) mucins were determined immunohistochemically in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae. Forty-seven-week-old colostrum-deprived pigs were randomly allocated to infected (n=20) or control groups (n=20). Five infected and uninfected pigs were euthanized at 0, 6, 12, and 48. h post-inoculation (hpi). In the infected pigs, the expression of both types of mucins, which were invariably observed, was associated with bronchiolar and respiratory bronchiolar lesions. Strong positive mucin signals were seen on the surface of bronchiolar and respiratory bronchiolar epithelium with neutrophil infiltration. The mean mucin-positive area peaked at 6. hpi and decreased significantly to control levels by 48. hpi on the surface of the bronchiolar and respiratory bronchiolar epithelium. Further studies are needed to establish the functional relationship between mucin expression and the host defense mechanism against A. pleuropneumoniae in the lungs of infected pigs.

KW - Actinobacillus pleuropneumoniae

KW - Immunohistochemistry

KW - Lung

KW - Mucin

KW - Pig

UR - https://www.scopus.com/pages/publications/84859108340

U2 - 10.1016/j.rvsc.2011.04.016

DO - 10.1016/j.rvsc.2011.04.016

M3 - Article

C2 - 21592537

AN - SCOPUS:84859108340

SN - 0034-5288

VL - 92

SP - 486

EP - 491

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 3

ER -

Expression of secreted mucins (MUC2, MUC5AC, MUC5B, and MUC6) and membrane-bound mucin (MUC4) in the lungs of pigs experimentally infected with Actinobacillus pleuropneumoniae

Abstract

Bibliographical note

Keywords

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