Extraction of β-carotene produced from yeast Rhodosporidium sp. and its heat stability

Jong Kook Kim; Jung In Kim; Nam Keun Lee; Young Tae Hahm; Moo Yeol Baik; Byung Yong Kim

doi:10.1007/s10068-010-0038-6

Extraction of β-carotene produced from yeast Rhodosporidium sp. and its heat stability

Jong Kook Kim, Jung In Kim, Nam Keun Lee, Young Tae Hahm, Moo Yeol Baik, Byung Yong Kim

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Abstract

This work determined the characteristics of β- carotene produced from Rhodosporidium sp. isolated from citrus fruits, and an effective extraction method was established. To extract β-carotene from the isolated Rhodosporidium sp., the cell walls were destroyed using dimethyl sulfoxide (DMSO) solution with and without the use of glass beads and a sonicator. Extracted β-carotene was identified by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) with a β-carotene standard. The yields of β- carotene extracted in DMSO, DMSO and glass beads, and DMSO with a sonicator were 3.371, 5.112, and 3.301 μg/ mL, respectively. Isolated β-carotene was relatively heat stable, with 80% of the viable molecules remaining at 80°C

Original language	English
Pages (from-to)	263-266
Number of pages	4
Journal	Food Science and Biotechnology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1007/s10068-010-0038-6
Publication status	Published - Feb 2010

Bibliographical note

Funding Information:
Acknowledgments This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (KRF-2007-314-F00010).

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title = "Extraction of β-carotene produced from yeast Rhodosporidium sp. and its heat stability",

abstract = "This work determined the characteristics of β- carotene produced from Rhodosporidium sp. isolated from citrus fruits, and an effective extraction method was established. To extract β-carotene from the isolated Rhodosporidium sp., the cell walls were destroyed using dimethyl sulfoxide (DMSO) solution with and without the use of glass beads and a sonicator. Extracted β-carotene was identified by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) with a β-carotene standard. The yields of β- carotene extracted in DMSO, DMSO and glass beads, and DMSO with a sonicator were 3.371, 5.112, and 3.301 μg/ mL, respectively. Isolated β-carotene was relatively heat stable, with 80% of the viable molecules remaining at 80°C",

author = "Kim, {Jong Kook} and Kim, {Jung In} and Lee, {Nam Keun} and Hahm, {Young Tae} and Baik, {Moo Yeol} and Kim, {Byung Yong}",

note = "Funding Information: Acknowledgments This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (KRF-2007-314-F00010).",

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doi = "10.1007/s10068-010-0038-6",

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AU - Kim, Jong Kook

AU - Kim, Jung In

AU - Lee, Nam Keun

AU - Hahm, Young Tae

AU - Baik, Moo Yeol

AU - Kim, Byung Yong

N1 - Funding Information: Acknowledgments This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (KRF-2007-314-F00010).

PY - 2010/2

Y1 - 2010/2

N2 - This work determined the characteristics of β- carotene produced from Rhodosporidium sp. isolated from citrus fruits, and an effective extraction method was established. To extract β-carotene from the isolated Rhodosporidium sp., the cell walls were destroyed using dimethyl sulfoxide (DMSO) solution with and without the use of glass beads and a sonicator. Extracted β-carotene was identified by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) with a β-carotene standard. The yields of β- carotene extracted in DMSO, DMSO and glass beads, and DMSO with a sonicator were 3.371, 5.112, and 3.301 μg/ mL, respectively. Isolated β-carotene was relatively heat stable, with 80% of the viable molecules remaining at 80°C

AB - This work determined the characteristics of β- carotene produced from Rhodosporidium sp. isolated from citrus fruits, and an effective extraction method was established. To extract β-carotene from the isolated Rhodosporidium sp., the cell walls were destroyed using dimethyl sulfoxide (DMSO) solution with and without the use of glass beads and a sonicator. Extracted β-carotene was identified by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) with a β-carotene standard. The yields of β- carotene extracted in DMSO, DMSO and glass beads, and DMSO with a sonicator were 3.371, 5.112, and 3.301 μg/ mL, respectively. Isolated β-carotene was relatively heat stable, with 80% of the viable molecules remaining at 80°C

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M3 - Comment/debate

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SN - 1226-7708

VL - 19

SP - 263

EP - 266

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

IS - 1

ER -

Extraction of β-carotene produced from yeast Rhodosporidium sp. and its heat stability

Abstract

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