TY - GEN
T1 - Fabrication of a circular pdms microchannel to construct 3d culture microsystem
AU - Choi, Jong Seob
AU - Piao, Yunxian
AU - Seo, Tae Seok
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - In this study, we described the fabrication method for microfluidic channels with circular-cross sectional geometries by reflow phenomenon. The photoresist reflow leads to shape change from rectangular to semicircular, and we optimized the reflow conditions by using positive photoresist to form the perfect cylindrical micropatterns. The resultant circular micropattern was used as a template for generating PDMS circular microchannels. The channel routes could be easily modified for mimicking the vascular network. To generate a 3D endothelial cell layer, we cultured the HUVECs inside the PDMS circular microchannels, and monitored the adhesion, proliferation, and alignment of HUVEC to the direction of the microchannel.
AB - In this study, we described the fabrication method for microfluidic channels with circular-cross sectional geometries by reflow phenomenon. The photoresist reflow leads to shape change from rectangular to semicircular, and we optimized the reflow conditions by using positive photoresist to form the perfect cylindrical micropatterns. The resultant circular micropattern was used as a template for generating PDMS circular microchannels. The channel routes could be easily modified for mimicking the vascular network. To generate a 3D endothelial cell layer, we cultured the HUVECs inside the PDMS circular microchannels, and monitored the adhesion, proliferation, and alignment of HUVEC to the direction of the microchannel.
KW - Cell culture
KW - Circular microchannels
KW - Human umbilical vein endothelial cell (HUVEC)
KW - Polydimethylsiloxane (PDMS)
KW - Reflow of photoresist
UR - http://www.scopus.com/inward/record.url?scp=84901753249&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901753249
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 485
EP - 487
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -