Flavobacterium tyrosinilyticum sp. Nov., isolated from the rhizosphere of wild strawberry

A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterial strain, designated THG-DN8.8^T, was isolated from the rhizosphere of a wild strawberry plant, located on Gyeryong Mountain, Daejeon, Republic of Korea. According to 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Flavobacterium and was related most closely to Flavobacterium banpakuense 15F3^T(97.7 % sequence similarity), Flavobacterium ginsenosidimutans THG-01^T(97.3 %), Flavobacterium ginsengiterrae DCY55^T(96.9 %) and Flavobacterium chungbukense CS100^T(96.7 %). Catalase and oxidase tests were positive. Levels of DNA–DNA relatedness with its phylogenetically closest neighbours were below 40.0 %. The DNA G+C content was determined to be 39.1 mol%. Strain THG-DN8.8^Talso was found to be able to grow at 4–33 *#X00B0;C, with 0–2 % (w/v) NaCl and at pH 5.5–9.5. The major fatty acids were iso-C_{15: 0}, summed feature 3 (comprising C_{16: 1}ω7c and/or C_{16: 1}ω6c) and C_{16: 0}. Menaquinone-6 (MK-6) was the dominant respiratory quinone, and phosphatidylethanolamine and phosphatidyldiethanolamine were the main polar lipids. On the basis of the data presented, strain THG-DN8.8^Trepresents a novel species, for which the name Flavobacterium tyrosinilyticum sp. nov. is proposed. The type strain is THG-DN8.8^T(=KCTC 42726^T=CCTCC AB 2015295^T).