Fluorescence-intensity multiplexing using fluorescent silica nanoparticles in a sheathless microchip flow cytometer

H. Yun, H. Bang, W. G. Lee, J. Min, T. G. Park, C. Chung, D. C. Han

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Conventional flow cytometers typically require multiple detectors for simultaneous identification of multiple subsets of immune cells. To minimize the number of detectors in portable flow cytometry or to analyze multi-parametric cellular information with minimum number of detectors in conventional flow cytometers, we propose a versatile multiplexed cell-counting method using functional silica nanoparticles (SiNPs). Fluorescein-5-isothiocyanate (FITC)-doped SiNPs, which are 100 times brighter than the FITC molecules itself, were used as new intensity-based fluorescent dye complexes to measure two subsets of leukocytes using a single detector simultaneously.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages121-123
Number of pages3
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 3 Oct 20107 Oct 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume1

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period3/10/107/10/10

Keywords

  • CD4 T-cells
  • Fluorescent silica nanoparticle
  • Microchip flow cytometry
  • Sheathless

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