Fluorogenic assay of alkaline phosphatase activity based on the modulation of excited-state intramolecular proton transfer

Jihye Park, Aasif Helal, Hong Seok Kim, Youngmi Kim

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A new fluorogenic substrate 1, which enables the fast and quantitative analysis of alkaline phosphatase activity, has been developed. Selective enzymatic hydrolysis of 1 instantly generated fluorescent compound 2 in aqueous media, which undergo an excited-state intramolecular proton transfer process, resulting in a remarkable fluorescence turn-on signal with an unusually large Stokes shift.

Original languageEnglish
Pages (from-to)5541-5544
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume22
Issue number17
DOIs
Publication statusPublished - 1 Sep 2012

Keywords

  • Alkaline phosphatase
  • Excited-state intramolecular proton transfer
  • Fluorogenic assay
  • Inhibitor assay
  • Probe

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