Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea: Emergence of clone ST131, 2006-2008

Hyung Seok Yang; Youngjin Kim; Sun Young Cho; You Sun Nam; Tae Sang Oh; Kyung Sun Park; Hyun Chul Kim; Hee Joo Lee

Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea: Emergence of clone ST131, 2006-2008

Hyung Seok Yang, Youngjin Kim, Sun Young Cho, You Sun Nam, Tae Sang Oh, Kyung Sun Park, Hyun Chul Kim, Hee Joo Lee

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Abstract

To investigate the sequence types (STs) and fluoroquinolone resistance related mutations among ciprofloxacin (CIP)-non-susceptible extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from Korean patients from 2006-2008. The prevalence of fluoroquinolone resistance-determining region (QRDR) mutations in gyrA, gyrB, parC, and parE and plasmid-mediated quinolone resistance (PMQR) genes were also studied. Multilocus sequence typing (MLST) was performed to identify STs. The most common ST was ST131 (33/51, 64.7%). All isolates, except one isolate, showed three mutations at codons 83 (S83L) and 87 (S87N) in gyrA and 80 (S80I) in parC. The prevalence of ST131 in our hospital was much higher than reported in other Asian studies during a similar time period. The mutations found in ST131 were concordant with other studies.

Original language	English
Pages (from-to)	511-515
Number of pages	5
Journal	Annals of Clinical and Laboratory Science
Volume	47
Issue number	4
Publication status	Published - 1 Jul 2017

Bibliographical note

Publisher Copyright:
© 2017 by the Association of Clinical Scientists, Inc.

Keywords

Ciprofloxacin resistance
ESBL
MLST
PMQR
QRDRs

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Yang, H. S., Kim, Y., Cho, S. Y., Nam, Y. S., Oh, T. S., Park, K. S., Kim, H. C., & Lee, H. J. (2017). Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea: Emergence of clone ST131, 2006-2008. Annals of Clinical and Laboratory Science, 47(4), 511-515.

Yang, Hyung Seok ; Kim, Youngjin ; Cho, Sun Young et al. / Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea : Emergence of clone ST131, 2006-2008. In: Annals of Clinical and Laboratory Science. 2017 ; Vol. 47, No. 4. pp. 511-515.

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title = "Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea: Emergence of clone ST131, 2006-2008",

abstract = "To investigate the sequence types (STs) and fluoroquinolone resistance related mutations among ciprofloxacin (CIP)-non-susceptible extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from Korean patients from 2006-2008. The prevalence of fluoroquinolone resistance-determining region (QRDR) mutations in gyrA, gyrB, parC, and parE and plasmid-mediated quinolone resistance (PMQR) genes were also studied. Multilocus sequence typing (MLST) was performed to identify STs. The most common ST was ST131 (33/51, 64.7%). All isolates, except one isolate, showed three mutations at codons 83 (S83L) and 87 (S87N) in gyrA and 80 (S80I) in parC. The prevalence of ST131 in our hospital was much higher than reported in other Asian studies during a similar time period. The mutations found in ST131 were concordant with other studies.",

keywords = "Ciprofloxacin resistance, ESBL, MLST, PMQR, QRDRs",

author = "Yang, {Hyung Seok} and Youngjin Kim and Cho, {Sun Young} and Nam, {You Sun} and Oh, {Tae Sang} and Park, {Kyung Sun} and Kim, {Hyun Chul} and Lee, {Hee Joo}",

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Yang, HS, Kim, Y , Cho, SY, Nam, YS, Oh, TS, Park, KS, Kim, HC & Lee, HJ 2017, 'Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea: Emergence of clone ST131, 2006-2008', Annals of Clinical and Laboratory Science, vol. 47, no. 4, pp. 511-515.

Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea: Emergence of clone ST131, 2006-2008. / Yang, Hyung Seok; Kim, Youngjin ; Cho, Sun Young et al.
In: Annals of Clinical and Laboratory Science, Vol. 47, No. 4, 01.07.2017, p. 511-515.

Research output: Contribution to journal › Article › peer-review

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T1 - Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea

T2 - Emergence of clone ST131, 2006-2008

AU - Yang, Hyung Seok

AU - Kim, Youngjin

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AU - Lee, Hee Joo

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AB - To investigate the sequence types (STs) and fluoroquinolone resistance related mutations among ciprofloxacin (CIP)-non-susceptible extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from Korean patients from 2006-2008. The prevalence of fluoroquinolone resistance-determining region (QRDR) mutations in gyrA, gyrB, parC, and parE and plasmid-mediated quinolone resistance (PMQR) genes were also studied. Multilocus sequence typing (MLST) was performed to identify STs. The most common ST was ST131 (33/51, 64.7%). All isolates, except one isolate, showed three mutations at codons 83 (S83L) and 87 (S87N) in gyrA and 80 (S80I) in parC. The prevalence of ST131 in our hospital was much higher than reported in other Asian studies during a similar time period. The mutations found in ST131 were concordant with other studies.

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Yang HS, Kim Y , Cho SY, Nam YS, Oh TS, Park KS et al. Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea: Emergence of clone ST131, 2006-2008. Annals of Clinical and Laboratory Science. 2017 Jul 1;47(4):511-515.

Fluoroquinolone resistance mechanisms by molecular epidemiologic study of ciprofloxacin-nonsusceptible Escherichia coli sequence types isolated from clinical specimens in a tertiary care university hospital in Korea: Emergence of clone ST131, 2006-2008

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Bibliographical note

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