Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root

Si Eun Lee; Eun Mi Ju; Jeong Hee Kim

doi:10.1038/emm.2001.43

Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root

Si Eun Lee
, Eun Mi Ju
, Jeong Hee Kim

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

The extract from Smilax china root has been used as medicinal remedy and reported to retain antimicrobial and antimutagenic acitivities. In this study, a possible presence of antioxidant activity of Smilax china root extract was investigated. Methanol extract (Me) revealed the presence of high 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC₅₀ 7.4 μg/ml) and protective property of cell's viability. Further fractionation with various solvent extraction and assay showed high levels of DPPH free radical scavenging activity in the ethyl acetate, butanol and water extracted fractions. In addition, V79-4 cells treated with Me of Smilax china root induced an increase of superoxide dismutase, catalase and glutathione peroxidase activities in a dose-dependent manner between 4-100 μg/ml. These results suggest that the medicinal component of the root of Smilax china extracts also contains antioxidant activity.

Original language	English
Pages (from-to)	263-268
Number of pages	6
Journal	Experimental and Molecular Medicine
Volume	33
Issue number	4
DOIs	https://doi.org/10.1038/emm.2001.43
Publication status	Published - 31 Dec 2001

Keywords

Antioxidant activity
Antioxidant enzymes
Lipid peroxidation
Radical scavenging
Smilax china root

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10.1038/emm.2001.43

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title = "Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root",

abstract = "The extract from Smilax china root has been used as medicinal remedy and reported to retain antimicrobial and antimutagenic acitivities. In this study, a possible presence of antioxidant activity of Smilax china root extract was investigated. Methanol extract (Me) revealed the presence of high 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50 7.4 μg/ml) and protective property of cell's viability. Further fractionation with various solvent extraction and assay showed high levels of DPPH free radical scavenging activity in the ethyl acetate, butanol and water extracted fractions. In addition, V79-4 cells treated with Me of Smilax china root induced an increase of superoxide dismutase, catalase and glutathione peroxidase activities in a dose-dependent manner between 4-100 μg/ml. These results suggest that the medicinal component of the root of Smilax china extracts also contains antioxidant activity.",

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author = "Lee, \{Si Eun\} and Ju, \{Eun Mi\} and Kim, \{Jeong Hee\}",

year = "2001",

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T1 - Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root

AU - Lee, Si Eun

AU - Ju, Eun Mi

AU - Kim, Jeong Hee

PY - 2001/12/31

Y1 - 2001/12/31

N2 - The extract from Smilax china root has been used as medicinal remedy and reported to retain antimicrobial and antimutagenic acitivities. In this study, a possible presence of antioxidant activity of Smilax china root extract was investigated. Methanol extract (Me) revealed the presence of high 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50 7.4 μg/ml) and protective property of cell's viability. Further fractionation with various solvent extraction and assay showed high levels of DPPH free radical scavenging activity in the ethyl acetate, butanol and water extracted fractions. In addition, V79-4 cells treated with Me of Smilax china root induced an increase of superoxide dismutase, catalase and glutathione peroxidase activities in a dose-dependent manner between 4-100 μg/ml. These results suggest that the medicinal component of the root of Smilax china extracts also contains antioxidant activity.

AB - The extract from Smilax china root has been used as medicinal remedy and reported to retain antimicrobial and antimutagenic acitivities. In this study, a possible presence of antioxidant activity of Smilax china root extract was investigated. Methanol extract (Me) revealed the presence of high 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50 7.4 μg/ml) and protective property of cell's viability. Further fractionation with various solvent extraction and assay showed high levels of DPPH free radical scavenging activity in the ethyl acetate, butanol and water extracted fractions. In addition, V79-4 cells treated with Me of Smilax china root induced an increase of superoxide dismutase, catalase and glutathione peroxidase activities in a dose-dependent manner between 4-100 μg/ml. These results suggest that the medicinal component of the root of Smilax china extracts also contains antioxidant activity.

KW - Antioxidant activity

KW - Antioxidant enzymes

KW - Lipid peroxidation

KW - Radical scavenging

KW - Smilax china root

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IS - 4

ER -

Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root

Abstract

Keywords

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