Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells

Hee Kyoung Jeon; Kyung Hwa Chang; Kyung Il Kim; In Sik Chung

doi:10.1023/A:1022330429508

Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells

Hee Kyoung Jeon
, Kyung Hwa Chang
, Kyung Il Kim
, In Sik Chung

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Recombinant tumstatin was expressed in stably transformed Drosophila melanogaster S2 cells and secreted into the medium with a molecular size of 29 kDa. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni²⁺ affinity fractionation. Purified recombinant tumstatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition for recombinant tumstatin was approx. 0.7 μg ml^-1. A maximum production of 4.6 μg recombinant tumstatin (10⁷ cells)^-1 was obtained in a T-flask culture of S2 cells, 7 d after induction with 0.5 mM CuSO₄.

Original language	English
Pages (from-to)	185-189
Number of pages	5
Journal	Biotechnology Letters
Volume	25
Issue number	3
DOIs	https://doi.org/10.1023/A:1022330429508
Publication status	Published - Feb 2003

Bibliographical note

Funding Information:
The authors thank the Kalluri laboratory for providing the human tumstatin cDNA construct. This work was supported by grants from the Brain Korea 21 project and from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University.

Keywords

Drosophila melanogaster
Endothelial cells
Recombinant protein
Tumstatin

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title = "Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells",

abstract = "Recombinant tumstatin was expressed in stably transformed Drosophila melanogaster S2 cells and secreted into the medium with a molecular size of 29 kDa. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni2+ affinity fractionation. Purified recombinant tumstatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition for recombinant tumstatin was approx. 0.7 μg ml-1. A maximum production of 4.6 μg recombinant tumstatin (107 cells)-1 was obtained in a T-flask culture of S2 cells, 7 d after induction with 0.5 mM CuSO4.",

keywords = "Drosophila melanogaster, Endothelial cells, Recombinant protein, Tumstatin",

author = "Jeon, \{Hee Kyoung\} and Chang, \{Kyung Hwa\} and Kim, \{Kyung Il\} and Chung, \{In Sik\}",

note = "Funding Information: The authors thank the Kalluri laboratory for providing the human tumstatin cDNA construct. This work was supported by grants from the Brain Korea 21 project and from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University.",

year = "2003",

month = feb,

doi = "10.1023/A:1022330429508",

language = "English",

volume = "25",

pages = "185--189",

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T1 - Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells

AU - Jeon, Hee Kyoung

AU - Chang, Kyung Hwa

AU - Kim, Kyung Il

AU - Chung, In Sik

N1 - Funding Information: The authors thank the Kalluri laboratory for providing the human tumstatin cDNA construct. This work was supported by grants from the Brain Korea 21 project and from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center, Kyung Hee University.

PY - 2003/2

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N2 - Recombinant tumstatin was expressed in stably transformed Drosophila melanogaster S2 cells and secreted into the medium with a molecular size of 29 kDa. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni2+ affinity fractionation. Purified recombinant tumstatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition for recombinant tumstatin was approx. 0.7 μg ml-1. A maximum production of 4.6 μg recombinant tumstatin (107 cells)-1 was obtained in a T-flask culture of S2 cells, 7 d after induction with 0.5 mM CuSO4.

AB - Recombinant tumstatin was expressed in stably transformed Drosophila melanogaster S2 cells and secreted into the medium with a molecular size of 29 kDa. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni2+ affinity fractionation. Purified recombinant tumstatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition for recombinant tumstatin was approx. 0.7 μg ml-1. A maximum production of 4.6 μg recombinant tumstatin (107 cells)-1 was obtained in a T-flask culture of S2 cells, 7 d after induction with 0.5 mM CuSO4.

KW - Drosophila melanogaster

KW - Endothelial cells

KW - Recombinant protein

KW - Tumstatin

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SN - 0141-5492

VL - 25

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EP - 189

JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells

Abstract

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Keywords

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