Functionalized quantum dots to quantify NADPH and their use for NADP+-dependent biocatalyzed transformations

Min Young Kim; Yea Seul Kim; Jisu Kim; Sang Soo Hah; Tae Jung Kim; Young Dong Kim

doi:10.1007/s10529-010-0476-5

Functionalized quantum dots to quantify NADPH and their use for NADP⁺-dependent biocatalyzed transformations

Min Young Kim
, Yea Seul Kim
, Jisu Kim
, Sang Soo Hah
, Tae Jung Kim
, Young Dong Kim

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Quantum dots are semiconducting nanoparticles that can be prepared with interesting optical properties. The fluorescent properties of quantum dots are one of the key advantages for their use as optical labels for biorecognition events and biocatalytic processes. We have prepared semiconductor quantum dots conjugated with Nile Blue (NB), and demonstrate that NB-functionalized quantum dots can act as versatile probes to analyze different biocatalyzed transformations, and can be used for the quantitative detection of NADPH as well as NADH. This approach provides a new path for the optical detection of NAD(P)H and for the quantitative analysis of NAD(P)⁺-dependent biotransformations.

Original language	English
Pages (from-to)	623-628
Number of pages	6
Journal	Biotechnology Letters
Volume	33
Issue number	3
DOIs	https://doi.org/10.1007/s10529-010-0476-5
Publication status	Published - Mar 2011

Bibliographical note

Funding Information:
Acknowledgments We are grateful to Professor Jin-Woo Bae for his help. This work was supported by Basic Science Program through the National Research Foundation of Korea (KRF) funded by the Ministry of Education, Science and Technology (MEST) (no. 2010-0015218) and also by the National Research Laboratory Fund through the Nano Optical Property Laboratory.

Keywords

Biocatalyzed transformation
Malic enzyme
NADPH determination
Quantum dots

Access to Document

10.1007/s10529-010-0476-5

Cite this

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title = "Functionalized quantum dots to quantify NADPH and their use for NADP+-dependent biocatalyzed transformations",

abstract = "Quantum dots are semiconducting nanoparticles that can be prepared with interesting optical properties. The fluorescent properties of quantum dots are one of the key advantages for their use as optical labels for biorecognition events and biocatalytic processes. We have prepared semiconductor quantum dots conjugated with Nile Blue (NB), and demonstrate that NB-functionalized quantum dots can act as versatile probes to analyze different biocatalyzed transformations, and can be used for the quantitative detection of NADPH as well as NADH. This approach provides a new path for the optical detection of NAD(P)H and for the quantitative analysis of NAD(P)+-dependent biotransformations.",

keywords = "Biocatalyzed transformation, Malic enzyme, NADPH determination, Quantum dots",

author = "Kim, \{Min Young\} and Kim, \{Yea Seul\} and Jisu Kim and Hah, \{Sang Soo\} and Kim, \{Tae Jung\} and Kim, \{Young Dong\}",

note = "Funding Information: Acknowledgments We are grateful to Professor Jin-Woo Bae for his help. This work was supported by Basic Science Program through the National Research Foundation of Korea (KRF) funded by the Ministry of Education, Science and Technology (MEST) (no. 2010-0015218) and also by the National Research Laboratory Fund through the Nano Optical Property Laboratory.",

year = "2011",

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doi = "10.1007/s10529-010-0476-5",

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AU - Kim, Min Young

AU - Kim, Yea Seul

AU - Kim, Jisu

AU - Hah, Sang Soo

AU - Kim, Tae Jung

AU - Kim, Young Dong

N1 - Funding Information: Acknowledgments We are grateful to Professor Jin-Woo Bae for his help. This work was supported by Basic Science Program through the National Research Foundation of Korea (KRF) funded by the Ministry of Education, Science and Technology (MEST) (no. 2010-0015218) and also by the National Research Laboratory Fund through the Nano Optical Property Laboratory.

PY - 2011/3

Y1 - 2011/3

N2 - Quantum dots are semiconducting nanoparticles that can be prepared with interesting optical properties. The fluorescent properties of quantum dots are one of the key advantages for their use as optical labels for biorecognition events and biocatalytic processes. We have prepared semiconductor quantum dots conjugated with Nile Blue (NB), and demonstrate that NB-functionalized quantum dots can act as versatile probes to analyze different biocatalyzed transformations, and can be used for the quantitative detection of NADPH as well as NADH. This approach provides a new path for the optical detection of NAD(P)H and for the quantitative analysis of NAD(P)+-dependent biotransformations.

AB - Quantum dots are semiconducting nanoparticles that can be prepared with interesting optical properties. The fluorescent properties of quantum dots are one of the key advantages for their use as optical labels for biorecognition events and biocatalytic processes. We have prepared semiconductor quantum dots conjugated with Nile Blue (NB), and demonstrate that NB-functionalized quantum dots can act as versatile probes to analyze different biocatalyzed transformations, and can be used for the quantitative detection of NADPH as well as NADH. This approach provides a new path for the optical detection of NAD(P)H and for the quantitative analysis of NAD(P)+-dependent biotransformations.

KW - Biocatalyzed transformation

KW - Malic enzyme

KW - NADPH determination

KW - Quantum dots

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