Generating photoperiod-sensitive genic male sterile rice lines with CRISPR/Cas9

Weihang Gu; Dabing Zhang; Yiping Qi; Zheng Yuan

doi:10.1007/978-1-4939-8991-1_8

Generating photoperiod-sensitive genic male sterile rice lines with CRISPR/Cas9

Weihang Gu, Dabing Zhang, Yiping Qi, Zheng Yuan

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

13 Citations (Scopus)

Abstract

Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both long-day and short-day conditions. The isolation and backcross process used for this could easily last for more than 3 years with a very low success rate. Here, we describe a straightforward method for generating csa-based PGMS lines by using the CRISPR-Cas9 technology in rice.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	97-107
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-8991-1_8
Publication status	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1917
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Agrobacterium-mediated transformation
CRISPR-Cas9
Carbon starved anther (csa)
Photoperiod-sensitive genic male sterility (PGMS)
Transgene-free
Two-line hybrid rice

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10.1007/978-1-4939-8991-1_8

Cite this

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abstract = "Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both long-day and short-day conditions. The isolation and backcross process used for this could easily last for more than 3 years with a very low success rate. Here, we describe a straightforward method for generating csa-based PGMS lines by using the CRISPR-Cas9 technology in rice.",

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AU - Gu, Weihang

AU - Zhang, Dabing

AU - Qi, Yiping

AU - Yuan, Zheng

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KW - Agrobacterium-mediated transformation

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KW - Carbon starved anther (csa)

KW - Photoperiod-sensitive genic male sterility (PGMS)

KW - Transgene-free

KW - Two-line hybrid rice

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T3 - Methods in Molecular Biology

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PB - Humana Press Inc.

ER -

Generating photoperiod-sensitive genic male sterile rice lines with CRISPR/Cas9

Abstract

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Keywords

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