Genes expression during osseointegration after various surface-treated dental implant installation

The present study was performed to examine the genes expression during osseointegration of dental implant. Machined, resorbable blast media(RBM), oxidized surface treated implants were used in this study. This 3 different surface treated implants with 5 mm in length and 3 mm in diameter were placed in the tibia of the rabbit. In the control group, only bone defect was made. Rabbits were sacrificed for histomophormetric evaluation and Gen-eFishing analysis at 1, 2, 6 weeks after implant placement. In histomorphometric evaluation with the measurement of BIC(bone-implant contact ratio) and BA(bone area ratio), RBM surface implant showed highest value in both BIC and BA, and machined surface implant showed lowest score. In GeneFishing™ screening analysis, 52 DEGs(differentially expressed genes) were expressed in 1 week group and 50 DEGs in 2 weeks group. This result suggests that implant surface treatment modulates the gene expression of bone tissue around dental implant. And then, direct sequencing and BLAST of 33 DEGs were performed. 6 DEGs were identified as known genes. Other DEGs were EST(expressed sequence tag) or unknown genes. This results suggests that many unknown genes are related to osseointegration of dental implant.