Genetic analysis and mapping of rice (Oryza sativa L.) male-sterile (OsMS-L) mutant

Haisheng Liu; Huangwei Chu; Hui Li; Hongmei Wang; Jiali Wei; Na Li; Shuyan Ding; Hai Huang; Hong Ma; Chaofeng Huang; Da Luo; Zheng Yuang; Jianhua Liu; Dabing Zhang

doi:10.1360/982004-423

Genetic analysis and mapping of rice (Oryza sativa L.) male-sterile (OsMS-L) mutant

Haisheng Liu
, Huangwei Chu
, Hui Li
, Hongmei Wang
, Jiali Wei
, Na Li
, Shuyan Ding
, Hai Huang
, Hong Ma
, Chaofeng Huang
, Da Luo
, Zheng Yuang
, Jianhua Liu
, Dabing Zhang

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with ⁶⁰Co γ-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells expanded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L locus, an F₂ population was constructed from the cross between the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.

Original language	English
Pages (from-to)	122-125
Number of pages	4
Journal	Chinese Science Bulletin
Volume	50
Issue number	2
DOIs	https://doi.org/10.1360/982004-423
Publication status	Published - 2005

Bibliographical note

Funding Information:
Acknowledgements We thank Mingjiao Chen, Zhijing Luo, Wei Ma for their help. This work was supported by The National Basic Research Program of China (Grant No. TG1999011605) and the Shanghai Municipal Committee of Science and Technology (Grant No. 03DJ14016).

Keywords

Gene mapping
Male-sterility
Molecular marker
Mutants
Rice (Oryza sativa L.)

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10.1360/982004-423

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@article{0062aaee503347ddbd74ba3acd8cdb85,

title = "Genetic analysis and mapping of rice (Oryza sativa L.) male-sterile (OsMS-L) mutant",

abstract = "A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with 60Co γ-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells expanded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L locus, an F2 population was constructed from the cross between the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.",

keywords = "Gene mapping, Male-sterility, Molecular marker, Mutants, Rice (Oryza sativa L.)",

author = "Haisheng Liu and Huangwei Chu and Hui Li and Hongmei Wang and Jiali Wei and Na Li and Shuyan Ding and Hai Huang and Hong Ma and Chaofeng Huang and Da Luo and Zheng Yuang and Jianhua Liu and Dabing Zhang",

note = "Funding Information: Acknowledgements We thank Mingjiao Chen, Zhijing Luo, Wei Ma for their help. This work was supported by The National Basic Research Program of China (Grant No. TG1999011605) and the Shanghai Municipal Committee of Science and Technology (Grant No. 03DJ14016).",

year = "2005",

doi = "10.1360/982004-423",

language = "English",

volume = "50",

pages = "122--125",

journal = "Chinese Science Bulletin",

issn = "1001-6538",

publisher = "Science Press",

number = "2",

}

TY - JOUR

T1 - Genetic analysis and mapping of rice (Oryza sativa L.) male-sterile (OsMS-L) mutant

AU - Liu, Haisheng

AU - Chu, Huangwei

AU - Li, Hui

AU - Wang, Hongmei

AU - Wei, Jiali

AU - Li, Na

AU - Ding, Shuyan

AU - Huang, Hai

AU - Ma, Hong

AU - Huang, Chaofeng

AU - Luo, Da

AU - Yuang, Zheng

AU - Liu, Jianhua

AU - Zhang, Dabing

N1 - Funding Information: Acknowledgements We thank Mingjiao Chen, Zhijing Luo, Wei Ma for their help. This work was supported by The National Basic Research Program of China (Grant No. TG1999011605) and the Shanghai Municipal Committee of Science and Technology (Grant No. 03DJ14016).

PY - 2005

Y1 - 2005

N2 - A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with 60Co γ-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells expanded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L locus, an F2 population was constructed from the cross between the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.

AB - A rice male-sterile mutant OsMS-L of japonica cultivar 9522 background, was obtained in M4 population treated with 60Co γ-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetum was retarded. Then tapetal cells expanded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To map OsMS-L locus, an F2 population was constructed from the cross between the OsMS-L (japonica) and LongTeFu B(indica). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM109 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for further fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.

KW - Gene mapping

KW - Male-sterility

KW - Molecular marker

KW - Mutants

KW - Rice (Oryza sativa L.)

UR - https://www.scopus.com/pages/publications/33749680150

U2 - 10.1360/982004-423

DO - 10.1360/982004-423

M3 - Article

AN - SCOPUS:33749680150

SN - 1001-6538

VL - 50

SP - 122

EP - 125

JO - Chinese Science Bulletin

JF - Chinese Science Bulletin

IS - 2

ER -

Genetic analysis and mapping of rice (Oryza sativa L.) male-sterile (OsMS-L) mutant

Abstract

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Keywords

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