TY - JOUR
T1 - Genetic diversity of zoonotic malaria parasites from mosquito vector and vertebrate hosts
AU - Wong, Meng Li
AU - Ahmed, Md Atique
AU - Sulaiman, Wan Yusoff Wan
AU - Manin, Benny O.
AU - Leong, Cherng Shii
AU - Quan, Fu Shi
AU - Chua, Tock H.
AU - Drakeley, Chris
AU - Snounou, Georges
AU - Vythilingam, Indra
N1 - Funding Information:
We would like to thank the staff of vector control programme Banggi Island, Sabah, and En. Mohd Fazreen Abdullah for the help they provided during the collection of mosquitoes. This work was supported by University of Malaya student Grant [IPP 105-2014A], Medical Research Council, Natural Environment Research Council, Economic and Social Research Council, and Biotechnology and Biosciences Research Council for the funding received for this project through the Environmental & Social Ecology of Human Infectious Diseases Initiative (ESEI), [MRC Grant Number: G1100796]. GS was supported by a grant from the Agence Nationale de la Recherche, France (ANR-17-CE13-0025-01). We declare there is no conflict of interest. Conceived and designed the experiments: IV, CD, THC; Funding acquisition: CD, IV; Performed the experiments: IV, MLW, CSL, WYWS, BOM; Analysed the data: MLW, AA, GS, FSQ; Wrote the paper: GS, AA, MLW, IV. All authors read and agreed to final version of manuscript. The authors declare no competing interests. All data are included in the manuscript and in the additional files.
Publisher Copyright:
© 2019
PY - 2019/9
Y1 - 2019/9
N2 - We explored and constructed haplotype network for simian malaria species: Plasmodium knowlesi, P. cynomolgi and P. inui aiming to understand the transmission dynamics between mosquitoes, humans and macaques. Mosquitoes were collected from villages in an area where zoonotic malaria is prevalent. PCR analysis confirmed Anopheles balabacensis as the main vector for macaque parasites, moreover nearly 60% of the mosquitoes harboured more than one Plasmodium species. Fragments of the A-type small subunit ribosomal RNA (SS rRNA) amplified from salivary gland sporozoites, and equivalent sequences obtained from GenBank were used to construct haplotype networks. The patterns were consistent with the presence of geographically distinct populations for P. inui and P. cynomolgi, and with three discrete P. knowlesi populations. This study provides a preliminary snapshot of the structure of these populations, that was insufficient to answer our aim. Thus, collection of parasites from their various hosts and over time, associated with a systematic analysis of a set of genetical loci is strongly advocated in order to obtain a clear picture of the parasite population and the flow between different hosts. This is important to devise measures that will minimise the risk of transmission to humans, because zoonotic malaria impedes malaria elimination.
AB - We explored and constructed haplotype network for simian malaria species: Plasmodium knowlesi, P. cynomolgi and P. inui aiming to understand the transmission dynamics between mosquitoes, humans and macaques. Mosquitoes were collected from villages in an area where zoonotic malaria is prevalent. PCR analysis confirmed Anopheles balabacensis as the main vector for macaque parasites, moreover nearly 60% of the mosquitoes harboured more than one Plasmodium species. Fragments of the A-type small subunit ribosomal RNA (SS rRNA) amplified from salivary gland sporozoites, and equivalent sequences obtained from GenBank were used to construct haplotype networks. The patterns were consistent with the presence of geographically distinct populations for P. inui and P. cynomolgi, and with three discrete P. knowlesi populations. This study provides a preliminary snapshot of the structure of these populations, that was insufficient to answer our aim. Thus, collection of parasites from their various hosts and over time, associated with a systematic analysis of a set of genetical loci is strongly advocated in order to obtain a clear picture of the parasite population and the flow between different hosts. This is important to devise measures that will minimise the risk of transmission to humans, because zoonotic malaria impedes malaria elimination.
KW - Anopheles balabacensis
KW - Haplotype
KW - Human
KW - Macaque
KW - Simian malaria parasites
UR - http://www.scopus.com/inward/record.url?scp=85064322688&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2019.04.010
DO - 10.1016/j.meegid.2019.04.010
M3 - Article
C2 - 30999059
AN - SCOPUS:85064322688
VL - 73
SP - 26
EP - 32
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
ER -
