Ginseng γ-thionin is localized to cell wall-bound extracellular spaces and responsive to biotic and abiotic stresses

Ok Ran Lee; Yu Jin Kim; Sri Renuka Devi Balusamy; Min Kyeoung Kim; Subramaniyam Sathiyamoorthy; Deok Chun Yang

doi:10.1016/j.pmpp.2011.05.004

Ginseng γ-thionin is localized to cell wall-bound extracellular spaces and responsive to biotic and abiotic stresses

Ok Ran Lee
, Yu Jin Kim
, Sri Renuka Devi Balusamy
, Min Kyeoung Kim
, Subramaniyam Sathiyamoorthy
, Deok Chun Yang

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Genomic DNA sequence of a γ-thionin in the Panax ginseng plant, termed Pgγ-thionin, contains 2 exons coding a 225-bp open reading frame with its deduced 75 amino acid sequence interrupted by 1 internal intron. The mRNA transcript level of Pgγ-thioninwas ubiquitously expressed in the leaf, flower bud, stem, root and rhizome, with its highest expression in the roots. Pgγ-thionin was responsive to both biotic and abiotic stresses, such as an 18-fold increase after exposure to ABA. Fluorescent tagged-Pgγ-thionin protein was localized to cell wall-bound extracellular spaces. Our finding indicates that Pgγ-thionin might be involved in cellular defense responses.

Original language	English
Pages (from-to)	82-89
Number of pages	8
Journal	Physiological and Molecular Plant Pathology
Volume	76
Issue number	2
DOIs	https://doi.org/10.1016/j.pmpp.2011.05.004
Publication status	Published - Aug 2011

Bibliographical note

Funding Information:
This study was supported by a grant from the Kyung Hee University in 2011. This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant # : PJ008204), Rural Development Administration, Republic of Korea.

Keywords

Abiotic stress
Biotic stress
Defense
Panax ginseng
Pathogen resistance
Thionin

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10.1016/j.pmpp.2011.05.004

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title = "Ginseng γ-thionin is localized to cell wall-bound extracellular spaces and responsive to biotic and abiotic stresses",

abstract = "Genomic DNA sequence of a γ-thionin in the Panax ginseng plant, termed Pgγ-thionin, contains 2 exons coding a 225-bp open reading frame with its deduced 75 amino acid sequence interrupted by 1 internal intron. The mRNA transcript level of Pgγ-thioninwas ubiquitously expressed in the leaf, flower bud, stem, root and rhizome, with its highest expression in the roots. Pgγ-thionin was responsive to both biotic and abiotic stresses, such as an 18-fold increase after exposure to ABA. Fluorescent tagged-Pgγ-thionin protein was localized to cell wall-bound extracellular spaces. Our finding indicates that Pgγ-thionin might be involved in cellular defense responses.",

keywords = "Abiotic stress, Biotic stress, Defense, Panax ginseng, Pathogen resistance, Thionin",

author = "Lee, \{Ok Ran\} and Kim, \{Yu Jin\} and Balusamy, \{Sri Renuka Devi\} and Kim, \{Min Kyeoung\} and Subramaniyam Sathiyamoorthy and Yang, \{Deok Chun\}",

note = "Funding Information: This study was supported by a grant from the Kyung Hee University in 2011. This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant \# : PJ008204), Rural Development Administration, Republic of Korea.",

year = "2011",

month = aug,

doi = "10.1016/j.pmpp.2011.05.004",

language = "English",

volume = "76",

pages = "82--89",

journal = "Physiological and Molecular Plant Pathology",

issn = "0885-5765",

publisher = "Academic Press",

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TY - JOUR

T1 - Ginseng γ-thionin is localized to cell wall-bound extracellular spaces and responsive to biotic and abiotic stresses

AU - Lee, Ok Ran

AU - Kim, Yu Jin

AU - Balusamy, Sri Renuka Devi

AU - Kim, Min Kyeoung

AU - Sathiyamoorthy, Subramaniyam

AU - Yang, Deok Chun

N1 - Funding Information: This study was supported by a grant from the Kyung Hee University in 2011. This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant # : PJ008204), Rural Development Administration, Republic of Korea.

PY - 2011/8

Y1 - 2011/8

N2 - Genomic DNA sequence of a γ-thionin in the Panax ginseng plant, termed Pgγ-thionin, contains 2 exons coding a 225-bp open reading frame with its deduced 75 amino acid sequence interrupted by 1 internal intron. The mRNA transcript level of Pgγ-thioninwas ubiquitously expressed in the leaf, flower bud, stem, root and rhizome, with its highest expression in the roots. Pgγ-thionin was responsive to both biotic and abiotic stresses, such as an 18-fold increase after exposure to ABA. Fluorescent tagged-Pgγ-thionin protein was localized to cell wall-bound extracellular spaces. Our finding indicates that Pgγ-thionin might be involved in cellular defense responses.

AB - Genomic DNA sequence of a γ-thionin in the Panax ginseng plant, termed Pgγ-thionin, contains 2 exons coding a 225-bp open reading frame with its deduced 75 amino acid sequence interrupted by 1 internal intron. The mRNA transcript level of Pgγ-thioninwas ubiquitously expressed in the leaf, flower bud, stem, root and rhizome, with its highest expression in the roots. Pgγ-thionin was responsive to both biotic and abiotic stresses, such as an 18-fold increase after exposure to ABA. Fluorescent tagged-Pgγ-thionin protein was localized to cell wall-bound extracellular spaces. Our finding indicates that Pgγ-thionin might be involved in cellular defense responses.

KW - Abiotic stress

KW - Biotic stress

KW - Defense

KW - Panax ginseng

KW - Pathogen resistance

KW - Thionin

UR - https://www.scopus.com/pages/publications/80455155097

U2 - 10.1016/j.pmpp.2011.05.004

DO - 10.1016/j.pmpp.2011.05.004

M3 - Article

AN - SCOPUS:80455155097

SN - 0885-5765

VL - 76

SP - 82

EP - 89

JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

IS - 2

ER -

Ginseng γ-thionin is localized to cell wall-bound extracellular spaces and responsive to biotic and abiotic stresses

Abstract

Bibliographical note

Keywords

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