Haladaptatus cibarius sp. nov., an extremely halophilic archaeon from seafood, and emended description of the genus Haladaptatus

A novel, extremely halophilic archaeon, D43^T, was isolated from traditional salt-fermented seafood in Korea. The cells were Gram-negative- staining and motile. The strain grew at 15-50 °C, 10-30% (w/v) NaCl and pH 6.0-8.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D43^T is affiliated with the family Halobacteriaceae in the domain Archaea and had 95.5% 16S rRNA gene sequence similarity with Haladaptatus paucihalophilus DX253^T. The sequence from strain D43^T formed a clade with those from Hap. paucihalophilus regardless of which tree-generating algorithm was used. DNA-DNA hybridization experiments showed 25.8% relatedness between the isolate and Hap. paucihalophilus KCTC 4006 ^T. Major lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two unidentified glycolipids. The DNA G+C content of the isolate was 56.5 mol%. On the basis of this polyphasic taxonomic study, strain D43^T represents a novel species in the genus Haladaptatus, for which the name Haladaptatus cibarius sp. nov. is proposed. The type strain is D43^T (=DSM 19505^T =JCM 15962^T).