Heat-treated Lactobacillus plantarum increases the immune responses through activation of natural killer cells and macrophages on in vivo and in vitro models

Phil Dong Moon, Jin Soo Lee, Hee Yun Kim, Na Ra Han, Inyeong Kang, Hyung Min Kim, Hyun Ja Jeong

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Purpose. Recently, Lactobacillus plantarum (nF1) has been reported to have immune-enhancing effects in an immunosuppressed- animal model. Natural killer (NK) cells and macrophages play important roles in the immune responses. However, immunomodulatory effects of heat-treated Lactobacillus plantarum-nF1 (hLp-nF1) on the activation of NK cells and macrophages have not been elucidated. Methodology. We assessed whether hLp-nF1 could elevate the activation of NK cells and macrophages using cyclophosphamide (CP)-induced immunosuppressed BALB/c mice and RAW 264.7 macrophages. A nitric oxide (NO) assay, enzyme-linked immunosorbent assay, Western blot analysis and NK cell activity assay were used to examine the effects of hLp-nF1 on the immune enhancement. Results/Key findings. Administration of hLp-nF1-elevated NK cell activities and serum levels of TNF-α, IL-2, and IL-12 in CPinduced immunosuppressed mice. In RAW 264.7 macrophages, treatment with hLp-nF1 increased the production of NO and expression of inducible NO synthase. Simultaneously, hLp-nF1 increased the production of TNF-α, IL-2, and IL-6 from RAW 264.7 cells. Finally, hLp-nF1 induced activation of nuclear factor-κB and phosphorylation of IκBα. Conclusion. We identified that hLp-nF1 has an immune-enhancing effect through the activation of NK cells and macrophages. Therefore, these findings suggest that hLp-nF1 would be helpful to enhance the immunity.

Original languageEnglish
Pages (from-to)467-474
Number of pages8
JournalJournal of Medical Microbiology
Volume68
Issue number3
DOIs
Publication statusPublished - Mar 2019

Keywords

  • Lactobacillus plantarum
  • Macrophages
  • Natural killer cells
  • Nitric oxide
  • Nuclear factor-κB

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