Hexane fractions of bupleurum falcatum L. stimulates glucagon-like peptide-1 secretion through gβγ -mediated pathway

Min Hee Shin; Eun Kyeong Choi; Ki Suk Kim; Kang Hoon Kim; Young Pyo Jang; Kwang Seok Ahn; Won Seok Chung; Nam Hyun Cha; Hyeung Jin Jang

doi:10.1155/2014/982165

Hexane fractions of bupleurum falcatum L. stimulates glucagon-like peptide-1 secretion through gβγ -mediated pathway

Min Hee Shin, Eun Kyeong Choi, Ki Suk Kim, Kang Hoon Kim, Young Pyo Jang, Kwang Seok Ahn, Won Seok Chung, Nam Hyun Cha, Hyeung Jin Jang

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Bupleurum falcatum L. has been used traditionally as a medicinal herb in Korean medicine. The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly. We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF. Through the microarray analysis, it was found that the ITPR2 gene that encodes the inositol 1,4,5-trisphosphate (IP receptor is up-regulated and the HFBF induces cell depolarization by inhibiting the voltage-gated channel expression in NCI-H716 cells. In addition, we found that the intracellular calcium in NCI-H716 cells, with Gallein, U73122, and 2APB as inhibitors, was decreased. These results suggest that the HFBF activates the GLP-1 secretion through the Gβγ pathways in the enteroendocrine L cells after treatment with the HFBF.

Original language	English
Article number	982165
Journal	Evidence-based Complementary and Alternative Medicine
Volume	2014
DOIs	https://doi.org/10.1155/2014/982165
Publication status	Published - 2014

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title = "Hexane fractions of bupleurum falcatum L. stimulates glucagon-like peptide-1 secretion through gβγ -mediated pathway",

abstract = "Bupleurum falcatum L. has been used traditionally as a medicinal herb in Korean medicine. The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly. We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF. Through the microarray analysis, it was found that the ITPR2 gene that encodes the inositol 1,4,5-trisphosphate (IP receptor is up-regulated and the HFBF induces cell depolarization by inhibiting the voltage-gated channel expression in NCI-H716 cells. In addition, we found that the intracellular calcium in NCI-H716 cells, with Gallein, U73122, and 2APB as inhibitors, was decreased. These results suggest that the HFBF activates the GLP-1 secretion through the Gβγ pathways in the enteroendocrine L cells after treatment with the HFBF.",

author = "Shin, {Min Hee} and Choi, {Eun Kyeong} and Kim, {Ki Suk} and Kim, {Kang Hoon} and Jang, {Young Pyo} and Ahn, {Kwang Seok} and Chung, {Won Seok} and Cha, {Nam Hyun} and Jang, {Hyeung Jin}",

year = "2014",

doi = "10.1155/2014/982165",

language = "English",

volume = "2014",

journal = "Evidence-based Complementary and Alternative Medicine",

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T1 - Hexane fractions of bupleurum falcatum L. stimulates glucagon-like peptide-1 secretion through gβγ -mediated pathway

AU - Shin, Min Hee

AU - Choi, Eun Kyeong

AU - Kim, Ki Suk

AU - Kim, Kang Hoon

AU - Jang, Young Pyo

AU - Ahn, Kwang Seok

AU - Chung, Won Seok

AU - Cha, Nam Hyun

AU - Jang, Hyeung Jin

PY - 2014

Y1 - 2014

N2 - Bupleurum falcatum L. has been used traditionally as a medicinal herb in Korean medicine. The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly. We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF. Through the microarray analysis, it was found that the ITPR2 gene that encodes the inositol 1,4,5-trisphosphate (IP receptor is up-regulated and the HFBF induces cell depolarization by inhibiting the voltage-gated channel expression in NCI-H716 cells. In addition, we found that the intracellular calcium in NCI-H716 cells, with Gallein, U73122, and 2APB as inhibitors, was decreased. These results suggest that the HFBF activates the GLP-1 secretion through the Gβγ pathways in the enteroendocrine L cells after treatment with the HFBF.

AB - Bupleurum falcatum L. has been used traditionally as a medicinal herb in Korean medicine. The hexane fraction of BF (HFBF), which was profiled with Direct Analysis in Real Time-Mass Spectrometry (DART-MS), activates the secretion of glucagon-like peptide-1 (GLP-1) in NCI-H716 cells significantly. We performed a microarray analysis and GLP-1 ELISA assay, as well as calcium imaging experiments with inhibitors, to investigate the mechanism of action of the HFBF. Through the microarray analysis, it was found that the ITPR2 gene that encodes the inositol 1,4,5-trisphosphate (IP receptor is up-regulated and the HFBF induces cell depolarization by inhibiting the voltage-gated channel expression in NCI-H716 cells. In addition, we found that the intracellular calcium in NCI-H716 cells, with Gallein, U73122, and 2APB as inhibitors, was decreased. These results suggest that the HFBF activates the GLP-1 secretion through the Gβγ pathways in the enteroendocrine L cells after treatment with the HFBF.

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DO - 10.1155/2014/982165

M3 - Article

AN - SCOPUS:84896138560

SN - 1741-427X

VL - 2014

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

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ER -

Hexane fractions of bupleurum falcatum L. stimulates glucagon-like peptide-1 secretion through gβγ -mediated pathway

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