Identification and metabolite profiling of alkaloids in aerial parts of Papaver rhoeas by liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry

Jae Hyeon Oh, In Jin Ha, Min Young Lee, Eun Ok Kim, Dain Park, Jun Hee Lee, Seok Geun Lee, Do Wan Kim, Tae Ho Lee, Eui Ju Lee, Chang Kug Kim

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Papaver plants can produce diverse bioactive alkaloids. Papaver rhoeas Linnaeus (common poppy or corn poppy) is an annual flowering medicinal plant used for treating cough, sleep disorder, and as a sedative, pain reliever, and food. It contains various powerful alkaloids like rhoeadine, benzylisoquinoline, and proaporphine. To investigate and identify alkaloids in the aerial parts of P. rhoeas, samples were collected at different growth stages and analyzed using liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. A liquid chromatography with mass spectrometry method was developed for the identification and metabolite profiling of alkaloids for P. rhoeas by comparing with Papaver somniferum. Eighteen alkaloids involved in benzylisoquinoline alkaloid biosynthesis were used to optimize the liquid chromatography gradient and mass spectrometry conditions. Fifty-five alkaloids, including protoberberine, benzylisoquinoline, aporphine, benzophenanthridine, and rhoeadine-type alkaloids, were identified authentically or tentatively by liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry in samples taken during various growth stages. Rhoeadine alkaloids were observed only in P. rhoeas samples, and codeine and morphine were tentatively identified in P. somniferum. The liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry method can be a powerful tool for the identification of diverse metabolites in the genus Papaver. These results may help understand the biosynthesis of alkaloids in P. rhoeas and evaluate the quality of this plant for possible medicinal applications.

Original languageEnglish
Pages (from-to)2517-2527
Number of pages11
JournalJournal of Separation Science
Volume41
Issue number12
DOIs
Publication statusPublished - Jun 2018

Bibliographical note

Publisher Copyright:
© 2018 The Authors. Journal of Separation Science Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

  • Papaver rhoeas
  • Papaver somniferum
  • alkaloids
  • liquid chromatography
  • metabolite profiling

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