Identification of a cis-acting regulatory motif recognized by MYB46, a master transcriptional regulator of secondary wall biosynthesis

Won Chan Kim; Jae Heung Ko; Kyung Hwan Han

doi:10.1007/s11103-012-9880-7

Identification of a cis-acting regulatory motif recognized by MYB46, a master transcriptional regulator of secondary wall biosynthesis

Won Chan Kim
, Jae Heung Ko
, Kyung Hwan Han

Research output: Contribution to journal › Article › peer-review

106 Citations (Scopus)

Abstract

While many aspects of primary cell wall have been extensively elucidated, our current understanding of secondary wall biosynthesis is limited. Recently, transcription factor MYB46 has been identified as a master regulator of secondary wall biosynthesis in Arabidopsis thaliana. To gain better understanding of this MYB46-mediated transcriptional regulation, we analyzed the promoter region of a direct target gene, AtC3H14, of MYB46 and identified a cis-acting regulatory motif that is recognized by MYB46. This MYB46-responsive cis-regulatory element (M46RE) was further characterized and shown to have an eight-nucleotide core motif, RKTWGGTR. We used electrophoretic mobility shift assay, transient transcriptional activation assay and chromatin immunoprecipitation analysis to show that the M46RE was necessary and sufficient for MYB46-responsive transcription. Genome-wide analysis identified that the frequency of M46RE in the promoters were highly enriched among the genes upregulated by MYB46, especially in the group of genes involved in secondary wall biosynthesis.

Original language	English
Pages (from-to)	489-501
Number of pages	13
Journal	Plant Molecular Biology
Volume	78
Issue number	4-5
DOIs	https://doi.org/10.1007/s11103-012-9880-7
Publication status	Published - Mar 2012

Bibliographical note

Funding Information:
Acknowledgments This work was funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DR-FC02-07ER64494). This project was also funded in part by a grant from the Michigan Agriculture Experiment Station and by the Ministry of Education, Science and Technology of Korea via the World Class University Project at Chonnam National University (R31-2009-000-20025-0) and by Basic Science Research Program through the National Research Foundation of Korea (NRF) (2011-0008840).

Keywords

MYB46
Secondary wall biosynthesis
Transcription factor
cis-acting motif

Access to Document

10.1007/s11103-012-9880-7

Cite this

@article{629af27b0d7548c1b10449a67ca33bc0,

title = "Identification of a cis-acting regulatory motif recognized by MYB46, a master transcriptional regulator of secondary wall biosynthesis",

abstract = "While many aspects of primary cell wall have been extensively elucidated, our current understanding of secondary wall biosynthesis is limited. Recently, transcription factor MYB46 has been identified as a master regulator of secondary wall biosynthesis in Arabidopsis thaliana. To gain better understanding of this MYB46-mediated transcriptional regulation, we analyzed the promoter region of a direct target gene, AtC3H14, of MYB46 and identified a cis-acting regulatory motif that is recognized by MYB46. This MYB46-responsive cis-regulatory element (M46RE) was further characterized and shown to have an eight-nucleotide core motif, RKTWGGTR. We used electrophoretic mobility shift assay, transient transcriptional activation assay and chromatin immunoprecipitation analysis to show that the M46RE was necessary and sufficient for MYB46-responsive transcription. Genome-wide analysis identified that the frequency of M46RE in the promoters were highly enriched among the genes upregulated by MYB46, especially in the group of genes involved in secondary wall biosynthesis.",

keywords = "MYB46, Secondary wall biosynthesis, Transcription factor, cis-acting motif",

author = "Kim, \{Won Chan\} and Ko, \{Jae Heung\} and Han, \{Kyung Hwan\}",

note = "Funding Information: Acknowledgments This work was funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DR-FC02-07ER64494). This project was also funded in part by a grant from the Michigan Agriculture Experiment Station and by the Ministry of Education, Science and Technology of Korea via the World Class University Project at Chonnam National University (R31-2009-000-20025-0) and by Basic Science Research Program through the National Research Foundation of Korea (NRF) (2011-0008840).",

year = "2012",

month = mar,

doi = "10.1007/s11103-012-9880-7",

language = "English",

volume = "78",

pages = "489--501",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer Science and Business Media B.V.",

number = "4-5",

}

TY - JOUR

T1 - Identification of a cis-acting regulatory motif recognized by MYB46, a master transcriptional regulator of secondary wall biosynthesis

AU - Kim, Won Chan

AU - Ko, Jae Heung

AU - Han, Kyung Hwan

N1 - Funding Information: Acknowledgments This work was funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DR-FC02-07ER64494). This project was also funded in part by a grant from the Michigan Agriculture Experiment Station and by the Ministry of Education, Science and Technology of Korea via the World Class University Project at Chonnam National University (R31-2009-000-20025-0) and by Basic Science Research Program through the National Research Foundation of Korea (NRF) (2011-0008840).

PY - 2012/3

Y1 - 2012/3

N2 - While many aspects of primary cell wall have been extensively elucidated, our current understanding of secondary wall biosynthesis is limited. Recently, transcription factor MYB46 has been identified as a master regulator of secondary wall biosynthesis in Arabidopsis thaliana. To gain better understanding of this MYB46-mediated transcriptional regulation, we analyzed the promoter region of a direct target gene, AtC3H14, of MYB46 and identified a cis-acting regulatory motif that is recognized by MYB46. This MYB46-responsive cis-regulatory element (M46RE) was further characterized and shown to have an eight-nucleotide core motif, RKTWGGTR. We used electrophoretic mobility shift assay, transient transcriptional activation assay and chromatin immunoprecipitation analysis to show that the M46RE was necessary and sufficient for MYB46-responsive transcription. Genome-wide analysis identified that the frequency of M46RE in the promoters were highly enriched among the genes upregulated by MYB46, especially in the group of genes involved in secondary wall biosynthesis.

AB - While many aspects of primary cell wall have been extensively elucidated, our current understanding of secondary wall biosynthesis is limited. Recently, transcription factor MYB46 has been identified as a master regulator of secondary wall biosynthesis in Arabidopsis thaliana. To gain better understanding of this MYB46-mediated transcriptional regulation, we analyzed the promoter region of a direct target gene, AtC3H14, of MYB46 and identified a cis-acting regulatory motif that is recognized by MYB46. This MYB46-responsive cis-regulatory element (M46RE) was further characterized and shown to have an eight-nucleotide core motif, RKTWGGTR. We used electrophoretic mobility shift assay, transient transcriptional activation assay and chromatin immunoprecipitation analysis to show that the M46RE was necessary and sufficient for MYB46-responsive transcription. Genome-wide analysis identified that the frequency of M46RE in the promoters were highly enriched among the genes upregulated by MYB46, especially in the group of genes involved in secondary wall biosynthesis.

KW - MYB46

KW - Secondary wall biosynthesis

KW - Transcription factor

KW - cis-acting motif

UR - https://www.scopus.com/pages/publications/84856880107

U2 - 10.1007/s11103-012-9880-7

DO - 10.1007/s11103-012-9880-7

M3 - Article

C2 - 22271306

AN - SCOPUS:84856880107

SN - 0167-4412

VL - 78

SP - 489

EP - 501

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 4-5

ER -

Identification of a cis-acting regulatory motif recognized by MYB46, a master transcriptional regulator of secondary wall biosynthesis

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this