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Identification of estrone in pomegranate (punica granatum) extracts by liquid chromatography-tandem mass spectrometry

  • Young Lim Kho
  • , Woong Jung
  • , Dohyeong Kwon
  • , Jung Hoan Kim

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

There were some reports that the pomegranate juice, seeds, and peel extracts contain steroid hormones including estrone, but the results were controversial. This study was performed to identify estrone in pomegranate extracts through a more sensitive and validated technology. Separation and determination of estrone was carried out using high performance liquid chromatography (HPLC) system with triple quadrupole mass spectrometry. Low concentration of estrone (4.5±0.38 ng/mL) was detected in pomegranate extracts with adequate precision value. The amount of estrone in pomegranate extracts was calculated as 16.4, 18.2, and 20.2 ng/mL, respectively, adjusted by extraction efficiency (average 25.6%) in the precision verification.

Original languageEnglish
Pages (from-to)809-813
Number of pages5
JournalFood Science and Biotechnology
Volume19
Issue number3
DOIs
Publication statusPublished - Jun 2010

Keywords

  • Estrogen
  • Estrone
  • Liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS)
  • Phytochemical
  • Pomegranate

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