Immobilized arylsulfotransferase

Dong Hyun Kim, Kyoichi Kobashi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Arylsulfotransferase was stabilized for storage more markedly by covalent immobilization onto AH-Sepharose-4B or CH-Sepharose -4B with EDC than by adsorptive immobilization onto DEAE-cellulose or DEAE-Sephadex. The optimal pH, Km for sulfate donor and thermostability of covalently immobilized arylsulfotransferase were similar to those of the free enzyme. Tyrosine-containing peptides such as cholecytokinin-8-nonsulfate, tyrosine methylester and (Leu)enkephalin as acceptor substrates were effectively sulfated by the immobilized enzyme.

Original languageEnglish
Pages (from-to)487-491
Number of pages5
JournalJournal of Biochemistry
Volume102
Issue number3
DOIs
Publication statusPublished - Sep 1987

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