Immunogenicity of low-pH treated whole viral influenza vaccine

Fu Shi Quan; Zhu Nan Li; Min Chul Kim; Dan Yang; Richard W. Compans; David A. Steinhauer; Sang Moo Kang

doi:10.1016/j.virol.2011.05.014

Immunogenicity of low-pH treated whole viral influenza vaccine

Fu Shi Quan
, Zhu Nan Li
, Min Chul Kim
, Dan Yang
, Richard W. Compans
, David A. Steinhauer
, Sang Moo Kang

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Low pH treatment of influenza virus hemagglutinin (HA) exposes its relatively conserved stalk domain, suggesting a potential immunogen with capability to induce broader immune responses. Here, we describe characterization, immunogenicity, antigenicity, and protective immunity induced by low pH treated inactivated whole viral vaccine in comparison with the untreated vaccine. The acidic pH treated viral vaccine showed high susceptibility to proteolytic cleavage and low hemagglutination activity indicating conformational changes. Immunization of mice with low pH treated viral vaccine induced lower levels of homologous or heterologous virus-specific binding and neutralizing antibodies compared to the untreated vaccine. Also, low pH treated influenza viral antigen showed lower antigenicity compared to the untreated influenza viral antigen. Lower efficacy of cross-protection against heterosubtypic virus was observed in the low-pH treated vaccine group. The results provide evidence that there is a correlation between protective efficacy and the stability of vaccines.

Original language	English
Pages (from-to)	196-202
Number of pages	7
Journal	Virology
Volume	417
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2011.05.014
Publication status	Published - 15 Aug 2011

Bibliographical note

Funding Information:
This work was supported in part by NIH/NIAID grant AI0680003 (R.W.C.), NIH/NIAID contract HHSN266200700006C (D.A.S.), the Georgia Research Alliance (S.M.K), and NIH/NIAID grants AI081385 (S.M.K.) and AI093772 (S.M.K.). The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the funding agents.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Conformational change
Immunogenicity
Influenza
Low pH
Vaccine

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10.1016/j.virol.2011.05.014

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title = "Immunogenicity of low-pH treated whole viral influenza vaccine",

abstract = "Low pH treatment of influenza virus hemagglutinin (HA) exposes its relatively conserved stalk domain, suggesting a potential immunogen with capability to induce broader immune responses. Here, we describe characterization, immunogenicity, antigenicity, and protective immunity induced by low pH treated inactivated whole viral vaccine in comparison with the untreated vaccine. The acidic pH treated viral vaccine showed high susceptibility to proteolytic cleavage and low hemagglutination activity indicating conformational changes. Immunization of mice with low pH treated viral vaccine induced lower levels of homologous or heterologous virus-specific binding and neutralizing antibodies compared to the untreated vaccine. Also, low pH treated influenza viral antigen showed lower antigenicity compared to the untreated influenza viral antigen. Lower efficacy of cross-protection against heterosubtypic virus was observed in the low-pH treated vaccine group. The results provide evidence that there is a correlation between protective efficacy and the stability of vaccines.",

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note = "Funding Information: This work was supported in part by NIH/NIAID grant AI0680003 (R.W.C.), NIH/NIAID contract HHSN266200700006C (D.A.S.), the Georgia Research Alliance (S.M.K), and NIH/NIAID grants AI081385 (S.M.K.) and AI093772 (S.M.K.). The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the funding agents.",

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AU - Kim, Min Chul

AU - Yang, Dan

AU - Compans, Richard W.

AU - Steinhauer, David A.

AU - Kang, Sang Moo

N1 - Funding Information: This work was supported in part by NIH/NIAID grant AI0680003 (R.W.C.), NIH/NIAID contract HHSN266200700006C (D.A.S.), the Georgia Research Alliance (S.M.K), and NIH/NIAID grants AI081385 (S.M.K.) and AI093772 (S.M.K.). The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the funding agents.

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KW - Low pH

KW - Vaccine

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Immunogenicity of low-pH treated whole viral influenza vaccine

Abstract

Bibliographical note

UN SDGs

Keywords

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