Immunohistochemical Study of Protein Kinase C in the Testes of Cattle and Pigs

We investigated the expression of novel protein kinase C (PKC) δ and θ in the testes of pigs and cattle using Western blot and immunohistochemical analysis. PKC δ and θ are recognized in the testes of pigs and cattle by Western blot analysis. We found in immunohistochemical study that PCK δ was localized in the spermatids of seminiferous tubules, but not in the interstitial cells, while PKC θ was recognized only in the interstitial cells of the testes of in both species. These findings suggest that PKC δ and θ play an important role in the development of spermatozoa and the regulation of androgen in the testicular interstitial cells (probably Leydig cells), respectively.