Improved indirubin production in a two-phase suspension culture of Polygonum tinctorium using dimethylpolysiloxane

G. W. Bae; C. S. Chung; K. I. Kim; C. H. Park; H. J. Lee; Y. A. Chae; I. S. Chung

doi:10.1023/A:1008800331295

Improved indirubin production in a two-phase suspension culture of Polygonum tinctorium using dimethylpolysiloxane

G. W. Bae
, C. S. Chung
, K. I. Kim
, C. H. Park
, H. J. Lee
, Y. A. Chae
, I. S. Chung

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Dimethylpolysiloxane (DMPS), used as a second phase in a suspension culture of Polygonum tinctorium, increased indirubin production by up to 259% and 16% in shake flask and air-lift bioreactors, respectively. In the shake flask culture, indirubin production increased by up to 248 mg/l using 90% v/v DMPS (volume of DMPS/volume of medium). In an air-lift bioreactor culture grown at a perfusion rate of 0.05 day^-1 the indirubin concentration reached a maximum of 130 mg/l using 50% (v/v) DMPS. For the same DMPS content, the lower indirubin production in an air-lift bioreactor was probably due to the mass transfer limitation of indirubin between the two phases.

Original language	English
Pages (from-to)	843-845
Number of pages	3
Journal	Biotechnology Techniques
Volume	12
Issue number	11
DOIs	https://doi.org/10.1023/A:1008800331295
Publication status	Published - 1998

Bibliographical note

Funding Information:
This work was supported by Grants from Kyung Hee University and the Korea Science and Engineering Foundation through Research Center for New Bio-Materials in Agriculture. We also gratefully acknowledge helpful comments from Prof. W.R. Curtis at Pennsylvania State University on this manuscript.

Keywords

Dimethylpolysiloxane
Indirubin
Polygonum tinctorium
Two-phase suspension culture

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10.1023/A:1008800331295

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@article{0a9a49dfa82847a5ac5f9fd29f07b975,

title = "Improved indirubin production in a two-phase suspension culture of Polygonum tinctorium using dimethylpolysiloxane",

abstract = "Dimethylpolysiloxane (DMPS), used as a second phase in a suspension culture of Polygonum tinctorium, increased indirubin production by up to 259\% and 16\% in shake flask and air-lift bioreactors, respectively. In the shake flask culture, indirubin production increased by up to 248 mg/l using 90\% v/v DMPS (volume of DMPS/volume of medium). In an air-lift bioreactor culture grown at a perfusion rate of 0.05 day-1 the indirubin concentration reached a maximum of 130 mg/l using 50\% (v/v) DMPS. For the same DMPS content, the lower indirubin production in an air-lift bioreactor was probably due to the mass transfer limitation of indirubin between the two phases.",

keywords = "Dimethylpolysiloxane, Indirubin, Polygonum tinctorium, Two-phase suspension culture",

author = "Bae, \{G. W.\} and Chung, \{C. S.\} and Kim, \{K. I.\} and Park, \{C. H.\} and Lee, \{H. J.\} and Chae, \{Y. A.\} and Chung, \{I. S.\}",

note = "Funding Information: This work was supported by Grants from Kyung Hee University and the Korea Science and Engineering Foundation through Research Center for New Bio-Materials in Agriculture. We also gratefully acknowledge helpful comments from Prof. W.R. Curtis at Pennsylvania State University on this manuscript.",

year = "1998",

doi = "10.1023/A:1008800331295",

language = "English",

volume = "12",

pages = "843--845",

journal = "Biotechnology Techniques",

issn = "0951-208X",

publisher = "Kluwer Academic Publishers",

number = "11",

}

TY - JOUR

T1 - Improved indirubin production in a two-phase suspension culture of Polygonum tinctorium using dimethylpolysiloxane

AU - Bae, G. W.

AU - Chung, C. S.

AU - Kim, K. I.

AU - Park, C. H.

AU - Lee, H. J.

AU - Chae, Y. A.

AU - Chung, I. S.

N1 - Funding Information: This work was supported by Grants from Kyung Hee University and the Korea Science and Engineering Foundation through Research Center for New Bio-Materials in Agriculture. We also gratefully acknowledge helpful comments from Prof. W.R. Curtis at Pennsylvania State University on this manuscript.

PY - 1998

Y1 - 1998

N2 - Dimethylpolysiloxane (DMPS), used as a second phase in a suspension culture of Polygonum tinctorium, increased indirubin production by up to 259% and 16% in shake flask and air-lift bioreactors, respectively. In the shake flask culture, indirubin production increased by up to 248 mg/l using 90% v/v DMPS (volume of DMPS/volume of medium). In an air-lift bioreactor culture grown at a perfusion rate of 0.05 day-1 the indirubin concentration reached a maximum of 130 mg/l using 50% (v/v) DMPS. For the same DMPS content, the lower indirubin production in an air-lift bioreactor was probably due to the mass transfer limitation of indirubin between the two phases.

AB - Dimethylpolysiloxane (DMPS), used as a second phase in a suspension culture of Polygonum tinctorium, increased indirubin production by up to 259% and 16% in shake flask and air-lift bioreactors, respectively. In the shake flask culture, indirubin production increased by up to 248 mg/l using 90% v/v DMPS (volume of DMPS/volume of medium). In an air-lift bioreactor culture grown at a perfusion rate of 0.05 day-1 the indirubin concentration reached a maximum of 130 mg/l using 50% (v/v) DMPS. For the same DMPS content, the lower indirubin production in an air-lift bioreactor was probably due to the mass transfer limitation of indirubin between the two phases.

KW - Dimethylpolysiloxane

KW - Indirubin

KW - Polygonum tinctorium

KW - Two-phase suspension culture

UR - https://www.scopus.com/pages/publications/0032436076

U2 - 10.1023/A:1008800331295

DO - 10.1023/A:1008800331295

M3 - Article

AN - SCOPUS:0032436076

SN - 0951-208X

VL - 12

SP - 843

EP - 845

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 11

ER -

Improved indirubin production in a two-phase suspension culture of Polygonum tinctorium using dimethylpolysiloxane

Abstract

Bibliographical note

Keywords

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