In vitro antiinflammatory activity of the essential oil extracted from Chrysanthemum sibiricum in murine macrophage RAW 264.7 cells

This research was undertaken to find the in vitro inflammatory action of the essenetial oil (CS-oil) extracted from Chrysanthemum sibiricum (Compositae) herbs. We investigated the effects of the CS-oil not only on the formation NO, PGE₂, and TNF-α but also on inducible nitric oxide synthase and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells. The data obtained were consistent with the modulation of iNOS enzyme expression. A similar fashion was also observed when LPS-induced PGE₂ release and COX-2 expression were tested. The significant inhibitory effects were shown in concentration-dependent manners. In addition, CS-oil also mildly but significantly reduced the formation of TNF-α. These findings support the application of CS-oil as an antiinflammatory essential oil.