Influenza immunization with trehalose-stabilized virus-like particle vaccine using microneedles

Yeu Chun Kim; Fu Shi Quan; Jae Min Song; Aswani Vunnava; Dae Goon Yoo; Kyoung Mi Park; Richard W. Compans; Sang Moo Kang; Mark R. Prausnitz

doi:10.1016/j.provac.2010.03.004

Influenza immunization with trehalose-stabilized virus-like particle vaccine using microneedles

Yeu Chun Kim
, Fu Shi Quan
, Jae Min Song
, Aswani Vunnava
, Dae Goon Yoo
, Kyoung Mi Park
, Richard W. Compans
, Sang Moo Kang
, Mark R. Prausnitz

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Morbidity and mortality due to seasonal and pandemic influenza could be reduced by simpler vaccination methods that enable improved vaccination coverage. In this study, solid metal microneedles coated with influenza virus-like particle (VLP) vaccine were inserted into skin for intradermal immunization. Microneedles were applied to the skin by hand and designed for simple administration with little or no training. Inclusion of trehalose in the coating formulation significantly increased vaccine stability during coating by maintaining hemagglutination activity. Mice vaccinated with stabilized microneedles developed strong antibody responses comparable to conventional intramuscular vaccination and were fully protected against subsequent viral challenge. Whereas, coating microneedles with a coating solution lacking trehalose led to only partial protection against lethal viral challenge. Therefore, our results show that microneedles coated with trehalose-stabilized VLP vaccine can be a promising tool for improving influenza vaccination.

Original language	English
Pages (from-to)	17-21
Number of pages	5
Journal	Procedia in Vaccinology
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.provac.2010.03.004
Publication status	Published - 2010

Bibliographical note

Funding Information:
The project described here was supported in part by NIH grants R01-EB006369 (M.R.P.), U01-AI0680003 (R.W.C.), SERCEB (R.W.C) and the Georgia Research Alliance (S.M.K). We thank Dr. Vladimir Zarnitsyn for microneedle fabrication and Dr. Mark Allen for use of his laser microfabrication facilities.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/j.provac.2010.03.004

Cite this

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title = "Influenza immunization with trehalose-stabilized virus-like particle vaccine using microneedles",

abstract = "Morbidity and mortality due to seasonal and pandemic influenza could be reduced by simpler vaccination methods that enable improved vaccination coverage. In this study, solid metal microneedles coated with influenza virus-like particle (VLP) vaccine were inserted into skin for intradermal immunization. Microneedles were applied to the skin by hand and designed for simple administration with little or no training. Inclusion of trehalose in the coating formulation significantly increased vaccine stability during coating by maintaining hemagglutination activity. Mice vaccinated with stabilized microneedles developed strong antibody responses comparable to conventional intramuscular vaccination and were fully protected against subsequent viral challenge. Whereas, coating microneedles with a coating solution lacking trehalose led to only partial protection against lethal viral challenge. Therefore, our results show that microneedles coated with trehalose-stabilized VLP vaccine can be a promising tool for improving influenza vaccination.",

author = "Kim, \{Yeu Chun\} and Quan, \{Fu Shi\} and Song, \{Jae Min\} and Aswani Vunnava and Yoo, \{Dae Goon\} and Park, \{Kyoung Mi\} and Compans, \{Richard W.\} and Kang, \{Sang Moo\} and Prausnitz, \{Mark R.\}",

note = "Funding Information: The project described here was supported in part by NIH grants R01-EB006369 (M.R.P.), U01-AI0680003 (R.W.C.), SERCEB (R.W.C) and the Georgia Research Alliance (S.M.K). We thank Dr. Vladimir Zarnitsyn for microneedle fabrication and Dr. Mark Allen for use of his laser microfabrication facilities.",

year = "2010",

doi = "10.1016/j.provac.2010.03.004",

language = "English",

volume = "2",

pages = "17--21",

journal = "Procedia in Vaccinology",

issn = "1877-282X",

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T1 - Influenza immunization with trehalose-stabilized virus-like particle vaccine using microneedles

AU - Kim, Yeu Chun

AU - Quan, Fu Shi

AU - Song, Jae Min

AU - Vunnava, Aswani

AU - Yoo, Dae Goon

AU - Park, Kyoung Mi

AU - Compans, Richard W.

AU - Kang, Sang Moo

AU - Prausnitz, Mark R.

N1 - Funding Information: The project described here was supported in part by NIH grants R01-EB006369 (M.R.P.), U01-AI0680003 (R.W.C.), SERCEB (R.W.C) and the Georgia Research Alliance (S.M.K). We thank Dr. Vladimir Zarnitsyn for microneedle fabrication and Dr. Mark Allen for use of his laser microfabrication facilities.

PY - 2010

Y1 - 2010

N2 - Morbidity and mortality due to seasonal and pandemic influenza could be reduced by simpler vaccination methods that enable improved vaccination coverage. In this study, solid metal microneedles coated with influenza virus-like particle (VLP) vaccine were inserted into skin for intradermal immunization. Microneedles were applied to the skin by hand and designed for simple administration with little or no training. Inclusion of trehalose in the coating formulation significantly increased vaccine stability during coating by maintaining hemagglutination activity. Mice vaccinated with stabilized microneedles developed strong antibody responses comparable to conventional intramuscular vaccination and were fully protected against subsequent viral challenge. Whereas, coating microneedles with a coating solution lacking trehalose led to only partial protection against lethal viral challenge. Therefore, our results show that microneedles coated with trehalose-stabilized VLP vaccine can be a promising tool for improving influenza vaccination.

AB - Morbidity and mortality due to seasonal and pandemic influenza could be reduced by simpler vaccination methods that enable improved vaccination coverage. In this study, solid metal microneedles coated with influenza virus-like particle (VLP) vaccine were inserted into skin for intradermal immunization. Microneedles were applied to the skin by hand and designed for simple administration with little or no training. Inclusion of trehalose in the coating formulation significantly increased vaccine stability during coating by maintaining hemagglutination activity. Mice vaccinated with stabilized microneedles developed strong antibody responses comparable to conventional intramuscular vaccination and were fully protected against subsequent viral challenge. Whereas, coating microneedles with a coating solution lacking trehalose led to only partial protection against lethal viral challenge. Therefore, our results show that microneedles coated with trehalose-stabilized VLP vaccine can be a promising tool for improving influenza vaccination.

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SN - 1877-282X

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JO - Procedia in Vaccinology

JF - Procedia in Vaccinology

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Influenza immunization with trehalose-stabilized virus-like particle vaccine using microneedles

Abstract

Bibliographical note

UN SDGs

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