Inhibition of airway MUC5AC mucin production and gene expression induced by epidermal growth factor or phorbol ester by glycyrrhizin and carbenoxolone

Hyun Jae Lee, Su Yel Lee, Heung Seog Bae, Jang Hyun Kim, Gyu Tae Chang, Jeong Ho Seok, Choong Jae Lee

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Background and aim: In this study, we investigated whether glycyrrhizin and carbenoxolone affect MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF) or phorbol ester (PMA) from human airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with EGF and PMA for 24 h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. Results: Glycyrrhizin and carbenoxolone were found to inhibit the production of MUC5AC mucin protein induced by EGF or PMA, and both compounds also inhibited the expression of MUC5AC mucin gene induced by EGF or PMA. Conclusion: These results suggest that glycyrrhizin and carbenoxolone can inhibit mucin gene expression and production of mucin protein, by directly acting on airway epithelial cells.

Original languageEnglish
Pages (from-to)743-747
Number of pages5
JournalPhytomedicine
Volume18
Issue number8-9
DOIs
Publication statusPublished - 15 Jun 2011

Bibliographical note

Funding Information:
This study was partly supported by 2009 grant of Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

Keywords

  • Airway mucin
  • Carbenoxolone
  • Glycyrrhizin
  • MUC5AC gene

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