Inhibition of cell proliferation and migration by miR-509-3p that targets CDK2, Rac1, and PIK3C2A

Sena Yoon; Eunji Han; Young Chul Choi; Honghwan Kee; Yongsu Jeong; Jaeseung Yoon; Kwanghee Baek

doi:10.14348/molcells.2014.2360

Inhibition of cell proliferation and migration by miR-509-3p that targets CDK2, Rac1, and PIK3C2A

Sena Yoon, Eunji Han, Young Chul Choi, Honghwan Kee, Yongsu Jeong, Jaeseung Yoon, Kwanghee Baek

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3′-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.

Original language	English
Pages (from-to)	314-321
Number of pages	8
Journal	Molecules and Cells
Volume	37
Issue number	4
DOIs	https://doi.org/10.14348/molcells.2014.2360
Publication status	Published - Apr 2014

Keywords

CDK2
Growth inhibition
PIK3C2A
Rac1
miR-509-3p
microRNA

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.14348/molcells.2014.2360

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title = "Inhibition of cell proliferation and migration by miR-509-3p that targets CDK2, Rac1, and PIK3C2A",

abstract = "CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3′-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.",

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author = "Sena Yoon and Eunji Han and Choi, {Young Chul} and Honghwan Kee and Yongsu Jeong and Jaeseung Yoon and Kwanghee Baek",

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AU - Yoon, Sena

AU - Han, Eunji

AU - Choi, Young Chul

AU - Kee, Honghwan

AU - Jeong, Yongsu

AU - Yoon, Jaeseung

AU - Baek, Kwanghee

PY - 2014/4

Y1 - 2014/4

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AB - CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3′-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.

KW - CDK2

KW - Growth inhibition

KW - PIK3C2A

KW - Rac1

KW - miR-509-3p

KW - microRNA

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Inhibition of cell proliferation and migration by miR-509-3p that targets CDK2, Rac1, and PIK3C2A

Abstract

Keywords

UN SDGs

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