Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells

Cheol Soo Park; Young Choon Lee; Jong Dae Kim; Hyung Min Kim; Cheorl Ho Kim

doi:10.1016/j.vph.2004.01.003

Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells

Cheol Soo Park
, Young Choon Lee
, Jong Dae Kim
, Hyung Min Kim
, Cheorl Ho Kim

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

The pharmacological effects of Polygonum cuspidatum water extract (PCWE) on lipid biosynthesis were investigated in cultured human hepatocyte HepG2 cells. The addition of PCWE (5 and 20 μg/ml), which had no effect on cell proliferation and cellular protein content, caused a marked decrease in the cellular cholesterol content, particularly, the cholesteryl ester content following 24 h of incubation. The incorporation of ¹⁴C-oleate into the cellular cholesteryl ester fraction was also reduced remarkably during incubation for 6 and 24 h. The effect of PCWE on acyl-coenzyme A-cholesterol acyltransferase (ACAT) activity were studied in vitro to explore the mechanism by which PCWE inhibits cholesterol ester formation. The data confirmed that PCWE, in a dose dependent manner, remarkably inhibits ACAT activity. Among the main active chemicals of P. cuspidatum, resveratrol, a kind of flavonoid, decreased ACAT activity in a dose-dependent manner from the level of 10 ^-3 M. Theses results strongly suggest that PCWE reduces the cholesteryl ester formation in human hepatocytes by inhibiting ACAT.

Original language	English
Pages (from-to)	279-284
Number of pages	6
Journal	Vascular Pharmacology
Volume	40
Issue number	6
DOIs	https://doi.org/10.1016/j.vph.2004.01.003
Publication status	Published - Jan 2004

Bibliographical note

Funding Information:
This work was, in part, supported by National Research Laboratory Program, KISTEP, MOST, Korea (No. M10203000024-02J0000-01300 to C.H.KIM).

Keywords

ACAT
Acyl-coenzyme A-cholesterol acyltransferase
C-oleic acid
Cholesteryl ester
HepG2 cell
LDL
LPDS
Low-density lipoprotein
PCWE
Polygonum cuspidatum
Polygonum cuspidatum water extract
Resveratrol
VLDL
Very-low-density lipoprotein

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10.1016/j.vph.2004.01.003

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@article{e9dbfa77d46f455f9acc06549312ad74,

title = "Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells",

abstract = "The pharmacological effects of Polygonum cuspidatum water extract (PCWE) on lipid biosynthesis were investigated in cultured human hepatocyte HepG2 cells. The addition of PCWE (5 and 20 μg/ml), which had no effect on cell proliferation and cellular protein content, caused a marked decrease in the cellular cholesterol content, particularly, the cholesteryl ester content following 24 h of incubation. The incorporation of 14C-oleate into the cellular cholesteryl ester fraction was also reduced remarkably during incubation for 6 and 24 h. The effect of PCWE on acyl-coenzyme A-cholesterol acyltransferase (ACAT) activity were studied in vitro to explore the mechanism by which PCWE inhibits cholesterol ester formation. The data confirmed that PCWE, in a dose dependent manner, remarkably inhibits ACAT activity. Among the main active chemicals of P. cuspidatum, resveratrol, a kind of flavonoid, decreased ACAT activity in a dose-dependent manner from the level of 10 -3 M. Theses results strongly suggest that PCWE reduces the cholesteryl ester formation in human hepatocytes by inhibiting ACAT.",

keywords = "ACAT, Acyl-coenzyme A-cholesterol acyltransferase, C-oleic acid, Cholesteryl ester, HepG2 cell, LDL, LPDS, Low-density lipoprotein, PCWE, Polygonum cuspidatum, Polygonum cuspidatum water extract, Resveratrol, VLDL, Very-low-density lipoprotein",

author = "Park, \{Cheol Soo\} and Lee, \{Young Choon\} and Kim, \{Jong Dae\} and Kim, \{Hyung Min\} and Kim, \{Cheorl Ho\}",

note = "Funding Information: This work was, in part, supported by National Research Laboratory Program, KISTEP, MOST, Korea (No. M10203000024-02J0000-01300 to C.H.KIM).",

year = "2004",

month = jan,

doi = "10.1016/j.vph.2004.01.003",

language = "English",

volume = "40",

pages = "279--284",

journal = "Vascular Pharmacology",

issn = "1537-1891",

publisher = "Elsevier Inc.",

number = "6",

}

Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells. / Park, Cheol Soo; Lee, Young Choon; Kim, Jong Dae et al.
In: Vascular Pharmacology, Vol. 40, No. 6, 01.2004, p. 279-284.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells

AU - Park, Cheol Soo

AU - Lee, Young Choon

AU - Kim, Jong Dae

AU - Kim, Hyung Min

AU - Kim, Cheorl Ho

N1 - Funding Information: This work was, in part, supported by National Research Laboratory Program, KISTEP, MOST, Korea (No. M10203000024-02J0000-01300 to C.H.KIM).

PY - 2004/1

Y1 - 2004/1

N2 - The pharmacological effects of Polygonum cuspidatum water extract (PCWE) on lipid biosynthesis were investigated in cultured human hepatocyte HepG2 cells. The addition of PCWE (5 and 20 μg/ml), which had no effect on cell proliferation and cellular protein content, caused a marked decrease in the cellular cholesterol content, particularly, the cholesteryl ester content following 24 h of incubation. The incorporation of 14C-oleate into the cellular cholesteryl ester fraction was also reduced remarkably during incubation for 6 and 24 h. The effect of PCWE on acyl-coenzyme A-cholesterol acyltransferase (ACAT) activity were studied in vitro to explore the mechanism by which PCWE inhibits cholesterol ester formation. The data confirmed that PCWE, in a dose dependent manner, remarkably inhibits ACAT activity. Among the main active chemicals of P. cuspidatum, resveratrol, a kind of flavonoid, decreased ACAT activity in a dose-dependent manner from the level of 10 -3 M. Theses results strongly suggest that PCWE reduces the cholesteryl ester formation in human hepatocytes by inhibiting ACAT.

AB - The pharmacological effects of Polygonum cuspidatum water extract (PCWE) on lipid biosynthesis were investigated in cultured human hepatocyte HepG2 cells. The addition of PCWE (5 and 20 μg/ml), which had no effect on cell proliferation and cellular protein content, caused a marked decrease in the cellular cholesterol content, particularly, the cholesteryl ester content following 24 h of incubation. The incorporation of 14C-oleate into the cellular cholesteryl ester fraction was also reduced remarkably during incubation for 6 and 24 h. The effect of PCWE on acyl-coenzyme A-cholesterol acyltransferase (ACAT) activity were studied in vitro to explore the mechanism by which PCWE inhibits cholesterol ester formation. The data confirmed that PCWE, in a dose dependent manner, remarkably inhibits ACAT activity. Among the main active chemicals of P. cuspidatum, resveratrol, a kind of flavonoid, decreased ACAT activity in a dose-dependent manner from the level of 10 -3 M. Theses results strongly suggest that PCWE reduces the cholesteryl ester formation in human hepatocytes by inhibiting ACAT.

KW - ACAT

KW - Acyl-coenzyme A-cholesterol acyltransferase

KW - C-oleic acid

KW - Cholesteryl ester

KW - HepG2 cell

KW - LDL

KW - LPDS

KW - Low-density lipoprotein

KW - PCWE

KW - Polygonum cuspidatum

KW - Polygonum cuspidatum water extract

KW - Resveratrol

KW - VLDL

KW - Very-low-density lipoprotein

UR - https://www.scopus.com/pages/publications/1842581940

U2 - 10.1016/j.vph.2004.01.003

DO - 10.1016/j.vph.2004.01.003

M3 - Article

C2 - 15063831

AN - SCOPUS:1842581940

SN - 1537-1891

VL - 40

SP - 279

EP - 284

JO - Vascular Pharmacology

JF - Vascular Pharmacology

IS - 6

ER -

Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme A-cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells

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Keywords

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