Integrated analysis of miRNA and mRNA expression profiles in human endothelial cells exposed to fisetin

MicroRNAs (miRNAs) play vital regulatory roles in various biological processes including cell differentiation, proliferation and apoptosis. Several studies have examined the expression profiles of miRNAs in response to different drugs and chemicals and suggested that expression patterns were associated with the effects of drugs and chemicals. Fisetin, a polyphenol found in a wide range of plants, has been reported to exhibit numerous biological activities, such as antioxidant, anti-inflammatory, antiangiogenic, and antiproliferative properties. In the present study, we evaluated whether fisetin regulates miRNA and mRNA expression. Using microarray analysis, we measured global miRNA and mRNA expression in human endothelial cells treated with 10 µM of fisetin for 24 h. Using several bioinformatic systems, we determined the signatures of potential biological processes and signaling pathways such as cell migration, apoptotic signaling pathway, cell motility, and cardiovascular system development. Our findings provide insight into the molecular mechanisms of action of fisetin and can be used to improve therapeutic intervention in vascular diseases.