Abstract
We have developed a direct loop mediated isothermal amplification (D-LAMP) microdevice incorporated with an immunochromatographic strip (ICS) for detecting bacteria in human whole blood. The fabrication and operation of the microdevice could be effectively simplified by utilizing a slidabe reaction chamber instead of using the microvalves and pumps for the fluid control. The D-LAMP technology does not require the cell lysis and purification step for amplifying DNA. Intact bacterial cells spiked in the whole blood was directly used as a template. Staphylococcus aureus (S. aureus, ATCC 12600) and Escherichia coli W (E. coli, ATCC 9637) in the whole blood were detected in the ICS without the sample pretreatment step within 70 min.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1238-1240 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
|---|---|
| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 25/10/15 → 29/10/15 |
Bibliographical note
Publisher Copyright:© 15CBMS-0001.
Keywords
- Bacteria detection
- Direct loop mediated isothermal amplification
- Immunochromato-graphic strip
- Slidable reaction chamber