@inproceedings{f5b7ed8b0ef34e1ab9d8794eccd5d2e1,
title = "Integrated polymerase chain reaction-capillary electrophoresis microdevice for hanwoo allele-specific genotyping",
abstract = "In this study, we developed a polymerase chain reaction-capillary electrophoresis (PCR-CE) microdevice to perform the allele-specific (AS) gene amplification and separation on a single wafer to identify Korean indigenous beef cattle, HANWOO. The HANWOO allele-specific genotyping system consists of HANWOO-specific five homozygous SNP sites, a sex-typing gene and a house-keeping gene as a control. The amplicons were produced in a 260 nL PCR chamber and then subsequently separated in a 6 cm-long CE microchannel to generate a DNA fingerprint of HANWOO. This on-site HANWOO genotyping platform can help the prompt prevention of zoonosis such as bovine spongiform encephalo-pathy (BSE) and efficient breed protection from imported beef cattle.",
keywords = "Allele-specific polymerase chain reaction, Capillary electrophoresis, HANWOO, SNP typing",
author = "Choi, {J. Y.} and Choi, {S. J.} and Y. Chen and Kim, {H. W.} and You, {S. A.} and Myeong, {H. K.} and Seo, {T. S.}",
year = "2010",
language = "English",
isbn = "9781618390622",
series = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
pages = "136--138",
booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",
}