Isolation and identification of phenolic compounds from the root bark of Morus alba L

The root barks of Morus alba L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and H₂O fractions. The repeated silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies of the EtOAc and n-BuOH fractions led to isolation of four phenolic compounds. The chemical structures of the compounds were determined as norartocarpanone (1), 2′,4′,7-trihydroxy-(2S)-flavanone (2), methyl β-resorcylate (3), and (Z)-oxyresveratrol-4-O-β-D-glucopyranoside (4). Compound 4 was isolated for the first time from the root barks of M. alba L.