Isomaltulose production via yeast surface display of sucrose isomerase from Enterobacter sp. FMB-1 on Saccharomyces cerevisiae

Gil Yong Lee; Jong Hyun Jung; Dong Ho Seo; Jantra Hansin; Suk Jin Ha; Jaeho Cha; Yong Sung Kim; Cheon Seok Park

doi:10.1016/j.biortech.2011.06.081

Isomaltulose production via yeast surface display of sucrose isomerase from Enterobacter sp. FMB-1 on Saccharomyces cerevisiae

Gil Yong Lee, Jong Hyun Jung, Dong Ho Seo, Jantra Hansin, Suk Jin Ha, Jaeho Cha, Yong Sung Kim, Cheon Seok Park

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

The gene encoding sucrose isomerase from Enterobacter sp. FMB-1 species (ESI) was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using a glycosylphosphatidylinositol (GPI) anchor attachment signal sequence. Fluorescence activated cell sorting (FACS) analysis and immunofluorescence microscopy confirmed the localization of ESI on the yeast cell surface. The displayed ESI (dESI) was stable at a broad range of temperatures (35-55 °C) and pHs (pH 5-7) with optimal temperature and pH at 45 °C and pH 7.0, respectively. In addition, the thermostability of the dESI was significantly enhanced compared with the recombinant ESI expressed in Escherichia coli. Biotransformation of sucrose to isomaltulose was observed in various ranges of substrate concentrations (50-250. mM) with a 6.4-7.4% conversion yield. It suggested that the bioconversion of sucrose to isomaltulose can be successfully performed by the dESI on the surface of host S. cerevisiae.

Original language	English
Pages (from-to)	9179-9184
Number of pages	6
Journal	Bioresource Technology
Volume	102
Issue number	19
DOIs	https://doi.org/10.1016/j.biortech.2011.06.081
Publication status	Published - Oct 2011

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2010-0028086).

Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.

Keywords

Enterobacter sp.
Isomaltulose
Saccharomyces cerevisiae
Sucrose isomerase
Surface display

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2011.06.081

Cite this

@article{595e00d0322d4b128a7a7d5775099abf,

title = "Isomaltulose production via yeast surface display of sucrose isomerase from Enterobacter sp. FMB-1 on Saccharomyces cerevisiae",

abstract = "The gene encoding sucrose isomerase from Enterobacter sp. FMB-1 species (ESI) was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using a glycosylphosphatidylinositol (GPI) anchor attachment signal sequence. Fluorescence activated cell sorting (FACS) analysis and immunofluorescence microscopy confirmed the localization of ESI on the yeast cell surface. The displayed ESI (dESI) was stable at a broad range of temperatures (35-55 °C) and pHs (pH 5-7) with optimal temperature and pH at 45 °C and pH 7.0, respectively. In addition, the thermostability of the dESI was significantly enhanced compared with the recombinant ESI expressed in Escherichia coli. Biotransformation of sucrose to isomaltulose was observed in various ranges of substrate concentrations (50-250. mM) with a 6.4-7.4% conversion yield. It suggested that the bioconversion of sucrose to isomaltulose can be successfully performed by the dESI on the surface of host S. cerevisiae.",

keywords = "Enterobacter sp., Isomaltulose, Saccharomyces cerevisiae, Sucrose isomerase, Surface display",

author = "Lee, {Gil Yong} and Jung, {Jong Hyun} and Seo, {Dong Ho} and Jantra Hansin and Ha, {Suk Jin} and Jaeho Cha and Kim, {Yong Sung} and Park, {Cheon Seok}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2010-0028086). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2011",

month = oct,

doi = "10.1016/j.biortech.2011.06.081",

language = "English",

volume = "102",

pages = "9179--9184",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd.",

number = "19",

}

TY - JOUR

T1 - Isomaltulose production via yeast surface display of sucrose isomerase from Enterobacter sp. FMB-1 on Saccharomyces cerevisiae

AU - Lee, Gil Yong

AU - Jung, Jong Hyun

AU - Seo, Dong Ho

AU - Hansin, Jantra

AU - Ha, Suk Jin

AU - Cha, Jaeho

AU - Kim, Yong Sung

AU - Park, Cheon Seok

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2010-0028086). Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2011/10

Y1 - 2011/10

N2 - The gene encoding sucrose isomerase from Enterobacter sp. FMB-1 species (ESI) was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using a glycosylphosphatidylinositol (GPI) anchor attachment signal sequence. Fluorescence activated cell sorting (FACS) analysis and immunofluorescence microscopy confirmed the localization of ESI on the yeast cell surface. The displayed ESI (dESI) was stable at a broad range of temperatures (35-55 °C) and pHs (pH 5-7) with optimal temperature and pH at 45 °C and pH 7.0, respectively. In addition, the thermostability of the dESI was significantly enhanced compared with the recombinant ESI expressed in Escherichia coli. Biotransformation of sucrose to isomaltulose was observed in various ranges of substrate concentrations (50-250. mM) with a 6.4-7.4% conversion yield. It suggested that the bioconversion of sucrose to isomaltulose can be successfully performed by the dESI on the surface of host S. cerevisiae.

AB - The gene encoding sucrose isomerase from Enterobacter sp. FMB-1 species (ESI) was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using a glycosylphosphatidylinositol (GPI) anchor attachment signal sequence. Fluorescence activated cell sorting (FACS) analysis and immunofluorescence microscopy confirmed the localization of ESI on the yeast cell surface. The displayed ESI (dESI) was stable at a broad range of temperatures (35-55 °C) and pHs (pH 5-7) with optimal temperature and pH at 45 °C and pH 7.0, respectively. In addition, the thermostability of the dESI was significantly enhanced compared with the recombinant ESI expressed in Escherichia coli. Biotransformation of sucrose to isomaltulose was observed in various ranges of substrate concentrations (50-250. mM) with a 6.4-7.4% conversion yield. It suggested that the bioconversion of sucrose to isomaltulose can be successfully performed by the dESI on the surface of host S. cerevisiae.

KW - Enterobacter sp.

KW - Isomaltulose

KW - Saccharomyces cerevisiae

KW - Sucrose isomerase

KW - Surface display

UR - http://www.scopus.com/inward/record.url?scp=80052362567&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2011.06.081

DO - 10.1016/j.biortech.2011.06.081

M3 - Article

C2 - 21803574

AN - SCOPUS:80052362567

SN - 0960-8524

VL - 102

SP - 9179

EP - 9184

JO - Bioresource Technology

JF - Bioresource Technology

IS - 19

ER -

Isomaltulose production via yeast surface display of sucrose isomerase from Enterobacter sp. FMB-1 on Saccharomyces cerevisiae

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this