Kunbi-Boshin-Hangam-Tang stimulates nitric oxide production through activation of nuclear factor-κB

H. N. Koo; H. J. Jeong; J. H. Park; G. Moon; H. J. Chae; H. R. Kim; C. H. Kim; S. B. Seo; N. H. An; H. M. Kim

doi:10.1081/IPH-100103858

Kunbi-Boshin-Hangam-Tang stimulates nitric oxide production through activation of nuclear factor-κB

H. N. Koo
, H. J. Jeong
, J. H. Park
, G. Moon
, H. J. Chae
, H. R. Kim
, C. H. Kim
, S. B. Seo
, N. H. An
, H. M. Kim

Research output: Contribution to journal › Article › peer-review

Abstract

The objective of the currently study was to determine the effect of Kunbi-Boshin-Hangam-Tang (KBH-Tang) on the production of nitric oxide (NO). Stimulation of RAW 264.7 cells with KBH-Tang after the treatment of recombinant interferon-γ (rIFN-γ) resulted in increased NO synthesis. KBH-Tang partially increased NO synthesis by itself. When KBH-Tang was used in combination with rIFN-γ, there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as increased amount of inducible NO synthase (iNOS) protein. NO production was inhibited by N^G-monomethyl-_L-arginine (N^GMMA). Furthermore, activation of nuclear factor (NF)-κB was increased by KBH-Tang. These results suggest that KBH-Tang may stimulate the NO production through the activation of the NF-κB.

Original language	English
Pages (from-to)	175-186
Number of pages	12
Journal	Immunopharmacology and Immunotoxicology
Volume	23
Issue number	2
DOIs	https://doi.org/10.1081/IPH-100103858
Publication status	Published - 2001

Bibliographical note

Funding Information:
This work was supported by Medicinal Resources Research Center of Wonk-wang University and partially by Wonkwang University in 2000.

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10.1081/IPH-100103858

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@article{fcdc9ea335b94f4690a52470c402ac80,

title = "Kunbi-Boshin-Hangam-Tang stimulates nitric oxide production through activation of nuclear factor-κB",

abstract = "The objective of the currently study was to determine the effect of Kunbi-Boshin-Hangam-Tang (KBH-Tang) on the production of nitric oxide (NO). Stimulation of RAW 264.7 cells with KBH-Tang after the treatment of recombinant interferon-γ (rIFN-γ) resulted in increased NO synthesis. KBH-Tang partially increased NO synthesis by itself. When KBH-Tang was used in combination with rIFN-γ, there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as increased amount of inducible NO synthase (iNOS) protein. NO production was inhibited by NG-monomethyl-L-arginine (NGMMA). Furthermore, activation of nuclear factor (NF)-κB was increased by KBH-Tang. These results suggest that KBH-Tang may stimulate the NO production through the activation of the NF-κB.",

author = "Koo, \{H. N.\} and Jeong, \{H. J.\} and Park, \{J. H.\} and G. Moon and Chae, \{H. J.\} and Kim, \{H. R.\} and Kim, \{C. H.\} and Seo, \{S. B.\} and An, \{N. H.\} and Kim, \{H. M.\}",

note = "Funding Information: This work was supported by Medicinal Resources Research Center of Wonk-wang University and partially by Wonkwang University in 2000.",

year = "2001",

doi = "10.1081/IPH-100103858",

language = "English",

volume = "23",

pages = "175--186",

journal = "Immunopharmacology and Immunotoxicology",

issn = "0892-3973",

publisher = "Taylor and Francis Ltd.",

number = "2",

}

TY - JOUR

T1 - Kunbi-Boshin-Hangam-Tang stimulates nitric oxide production through activation of nuclear factor-κB

AU - Koo, H. N.

AU - Jeong, H. J.

AU - Park, J. H.

AU - Moon, G.

AU - Chae, H. J.

AU - Kim, H. R.

AU - Kim, C. H.

AU - Seo, S. B.

AU - An, N. H.

AU - Kim, H. M.

N1 - Funding Information: This work was supported by Medicinal Resources Research Center of Wonk-wang University and partially by Wonkwang University in 2000.

PY - 2001

Y1 - 2001

N2 - The objective of the currently study was to determine the effect of Kunbi-Boshin-Hangam-Tang (KBH-Tang) on the production of nitric oxide (NO). Stimulation of RAW 264.7 cells with KBH-Tang after the treatment of recombinant interferon-γ (rIFN-γ) resulted in increased NO synthesis. KBH-Tang partially increased NO synthesis by itself. When KBH-Tang was used in combination with rIFN-γ, there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as increased amount of inducible NO synthase (iNOS) protein. NO production was inhibited by NG-monomethyl-L-arginine (NGMMA). Furthermore, activation of nuclear factor (NF)-κB was increased by KBH-Tang. These results suggest that KBH-Tang may stimulate the NO production through the activation of the NF-κB.

AB - The objective of the currently study was to determine the effect of Kunbi-Boshin-Hangam-Tang (KBH-Tang) on the production of nitric oxide (NO). Stimulation of RAW 264.7 cells with KBH-Tang after the treatment of recombinant interferon-γ (rIFN-γ) resulted in increased NO synthesis. KBH-Tang partially increased NO synthesis by itself. When KBH-Tang was used in combination with rIFN-γ, there was a marked cooperative induction of NO synthesis in a dose-dependent manner. This increase in NO synthesis was reflected as increased amount of inducible NO synthase (iNOS) protein. NO production was inhibited by NG-monomethyl-L-arginine (NGMMA). Furthermore, activation of nuclear factor (NF)-κB was increased by KBH-Tang. These results suggest that KBH-Tang may stimulate the NO production through the activation of the NF-κB.

UR - https://www.scopus.com/pages/publications/0034995531

U2 - 10.1081/IPH-100103858

DO - 10.1081/IPH-100103858

M3 - Article

C2 - 11417846

AN - SCOPUS:0034995531

SN - 0892-3973

VL - 23

SP - 175

EP - 186

JO - Immunopharmacology and Immunotoxicology

JF - Immunopharmacology and Immunotoxicology

IS - 2

ER -

Kunbi-Boshin-Hangam-Tang stimulates nitric oxide production through activation of nuclear factor-κB

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Bibliographical note

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