Lancemaside A isolated from Codonopsis lanceolata and its metabolite echinocystic acid ameliorate scopolamine-induced memory and learning deficits in mice

Il Hoon Jung; Se Eun Jang; Eun Ha Joh; Jayong Chung; Myung Joo Han; Dong Hyun Kim

doi:10.1016/j.phymed.2012.09.005

Lancemaside A isolated from Codonopsis lanceolata and its metabolite echinocystic acid ameliorate scopolamine-induced memory and learning deficits in mice

Il Hoon Jung, Se Eun Jang, Eun Ha Joh, Jayong Chung, Myung Joo Han, Dong Hyun Kim

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

The rhizome of Codonopsis lanceolata (family Campanulaceae), which contains lancemaside A as a main constituent, has been used as herbal medicine to treat inflammation, insomnia, and hypomnesia. Lancemaside A and echinocystic acid, which is its metabolite by intestinal microflora, potently inhibited acetylcholinesterase activity in a dose-dependent manner, with IC₅₀ value 13.6 μM and 12.2 μM, respectively. Its inhibitory potency is comparable with that of donepezil (IC₅₀ = 10.9 μM). Lancemaside A and echinocystic acid significantly reversed scopolamine-induced memory and learning deficits on passive avoidance task. Lancemaside A orally administered 5 h before treatment with scopolamine reversed scopolamine-induced memory and learning deficits more potently than one orally administered 1 h before. Echinocystic acid more potently reversed it than lancemaside A. Lancemaside A and echinocystic acid significantly reversed scopolamine-induced memory and learning deficits on the Y-maze and Morris water maze tasks. Lancemaside A and echinocystic acid also increased the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element binding protein (p-CREB). Based on these findings, orally administered lancemaside A may be metabolized to echinocystic acid, which may be absorbed into the blood and ameliorate memory and learning deficits by inhibiting AChE activity and inducing BDNF and p-CREB expressions.

Original language	English
Pages (from-to)	84-88
Number of pages	5
Journal	Phytomedicine
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/j.phymed.2012.09.005
Publication status	Published - 15 Dec 2012

Bibliographical note

Funding Information:
This study was supported by a grant from World Class University Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( R33-2008-000-10018-0 ).

Keywords

Acethylcholinesterase
Campanulaceae
Codonopsis lanceolata
Echinocystic acid
Lancemaside A
Memory

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10.1016/j.phymed.2012.09.005

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title = "Lancemaside A isolated from Codonopsis lanceolata and its metabolite echinocystic acid ameliorate scopolamine-induced memory and learning deficits in mice",

abstract = "The rhizome of Codonopsis lanceolata (family Campanulaceae), which contains lancemaside A as a main constituent, has been used as herbal medicine to treat inflammation, insomnia, and hypomnesia. Lancemaside A and echinocystic acid, which is its metabolite by intestinal microflora, potently inhibited acetylcholinesterase activity in a dose-dependent manner, with IC50 value 13.6 μM and 12.2 μM, respectively. Its inhibitory potency is comparable with that of donepezil (IC50 = 10.9 μM). Lancemaside A and echinocystic acid significantly reversed scopolamine-induced memory and learning deficits on passive avoidance task. Lancemaside A orally administered 5 h before treatment with scopolamine reversed scopolamine-induced memory and learning deficits more potently than one orally administered 1 h before. Echinocystic acid more potently reversed it than lancemaside A. Lancemaside A and echinocystic acid significantly reversed scopolamine-induced memory and learning deficits on the Y-maze and Morris water maze tasks. Lancemaside A and echinocystic acid also increased the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element binding protein (p-CREB). Based on these findings, orally administered lancemaside A may be metabolized to echinocystic acid, which may be absorbed into the blood and ameliorate memory and learning deficits by inhibiting AChE activity and inducing BDNF and p-CREB expressions.",

keywords = "Acethylcholinesterase, Campanulaceae, Codonopsis lanceolata, Echinocystic acid, Lancemaside A, Memory",

author = "Jung, {Il Hoon} and Jang, {Se Eun} and Joh, {Eun Ha} and Jayong Chung and Han, {Myung Joo} and Kim, {Dong Hyun}",

note = "Funding Information: This study was supported by a grant from World Class University Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( R33-2008-000-10018-0 ). ",

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doi = "10.1016/j.phymed.2012.09.005",

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T1 - Lancemaside A isolated from Codonopsis lanceolata and its metabolite echinocystic acid ameliorate scopolamine-induced memory and learning deficits in mice

AU - Jung, Il Hoon

AU - Jang, Se Eun

AU - Joh, Eun Ha

AU - Chung, Jayong

AU - Han, Myung Joo

AU - Kim, Dong Hyun

N1 - Funding Information: This study was supported by a grant from World Class University Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology ( R33-2008-000-10018-0 ).

PY - 2012/12/15

Y1 - 2012/12/15

N2 - The rhizome of Codonopsis lanceolata (family Campanulaceae), which contains lancemaside A as a main constituent, has been used as herbal medicine to treat inflammation, insomnia, and hypomnesia. Lancemaside A and echinocystic acid, which is its metabolite by intestinal microflora, potently inhibited acetylcholinesterase activity in a dose-dependent manner, with IC50 value 13.6 μM and 12.2 μM, respectively. Its inhibitory potency is comparable with that of donepezil (IC50 = 10.9 μM). Lancemaside A and echinocystic acid significantly reversed scopolamine-induced memory and learning deficits on passive avoidance task. Lancemaside A orally administered 5 h before treatment with scopolamine reversed scopolamine-induced memory and learning deficits more potently than one orally administered 1 h before. Echinocystic acid more potently reversed it than lancemaside A. Lancemaside A and echinocystic acid significantly reversed scopolamine-induced memory and learning deficits on the Y-maze and Morris water maze tasks. Lancemaside A and echinocystic acid also increased the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element binding protein (p-CREB). Based on these findings, orally administered lancemaside A may be metabolized to echinocystic acid, which may be absorbed into the blood and ameliorate memory and learning deficits by inhibiting AChE activity and inducing BDNF and p-CREB expressions.

AB - The rhizome of Codonopsis lanceolata (family Campanulaceae), which contains lancemaside A as a main constituent, has been used as herbal medicine to treat inflammation, insomnia, and hypomnesia. Lancemaside A and echinocystic acid, which is its metabolite by intestinal microflora, potently inhibited acetylcholinesterase activity in a dose-dependent manner, with IC50 value 13.6 μM and 12.2 μM, respectively. Its inhibitory potency is comparable with that of donepezil (IC50 = 10.9 μM). Lancemaside A and echinocystic acid significantly reversed scopolamine-induced memory and learning deficits on passive avoidance task. Lancemaside A orally administered 5 h before treatment with scopolamine reversed scopolamine-induced memory and learning deficits more potently than one orally administered 1 h before. Echinocystic acid more potently reversed it than lancemaside A. Lancemaside A and echinocystic acid significantly reversed scopolamine-induced memory and learning deficits on the Y-maze and Morris water maze tasks. Lancemaside A and echinocystic acid also increased the expression of brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element binding protein (p-CREB). Based on these findings, orally administered lancemaside A may be metabolized to echinocystic acid, which may be absorbed into the blood and ameliorate memory and learning deficits by inhibiting AChE activity and inducing BDNF and p-CREB expressions.

KW - Acethylcholinesterase

KW - Campanulaceae

KW - Codonopsis lanceolata

KW - Echinocystic acid

KW - Lancemaside A

KW - Memory

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U2 - 10.1016/j.phymed.2012.09.005

DO - 10.1016/j.phymed.2012.09.005

M3 - Article

C2 - 23079229

AN - SCOPUS:84870466589

SN - 0944-7113

VL - 20

SP - 84

EP - 88

JO - Phytomedicine

JF - Phytomedicine

IS - 1

ER -

Lancemaside A isolated from Codonopsis lanceolata and its metabolite echinocystic acid ameliorate scopolamine-induced memory and learning deficits in mice

Abstract

Bibliographical note

Keywords

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