Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant

Hina Singh; Juan Du; Hien T.T. Ngo; Kyung Hwa Won; Jung Eun Yang; Ki Young Kim; Tae Hoo Yi

doi:10.1007/s10482-015-0439-x

Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant

Hina Singh, Juan Du, Hien T.T. Ngo, Kyung Hwa Won, Jung Eun Yang, Ki Young Kim, Tae Hoo Yi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Two bacterial strains, designated THG-DN8.7^T and THG-DN8.3^T, were isolated from the rhizosphere of a strawberry plant in Gyeryong Mountain, South Korea. Cells of both isolates were observed to be Gram-negative, yellow-coloured and rod-shaped. Comparative 16S rRNA gene sequence analysis showed that strain THG-DN8.7^T had highest sequence similarities to Lysobacter yangpyeongensis KACC 11407^T (97.2 %), Lysobacter niabensis KACC 11587^T (97.0 %) and Lysobacter oryzae KCTC 22249^T (96.9 %), while strain THG-DN8.3^T had closely similarity with L. niabensis KACC 11587^T (98.1 %), L. oryzae KCTC 22249^T (97.1 %) and L. yangpyeongensis KACC 11407^T (96.1 %). DNA–DNA relatedness values between strains THG-DN8.7^T and THG-DN8.3^T and their closest phylogenetically neighbours were below 30.0 %, which indicates that strains THG-DN8.7^T and THG-DN8.3^T represent distinct species within the genus Lysobacter. Both strains were found to contain iso-C_15:0, iso-C_16:0 and iso-C_17:1ω9c as predominant fatty acids and ubiquinone-8 as major isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strains THG-DN8.7^T and THG-DN8.3^T were determined to be 66.9 and 67.8 mol%, respectively. These data are consistent with the affiliation of the two new species represented by THG-DN8.7^T and THG-DN8.3^T to the genus Lysobacter. The names Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. are proposed for these species with the type strains THG-DN8.7^T (=KCTC 42236^T = JCM 30322^T) and THG-DN8.3^T (=KCTC 42237^T = JCM 30321^T), respectively.

Original language	English
Article number	8
Pages (from-to)	1437-1444
Number of pages	8
Journal	Antonie van Leeuwenhoek
Volume	107
Issue number	6
DOIs	https://doi.org/10.1007/s10482-015-0439-x
Publication status	Published - 22 Jun 2015

Bibliographical note

Publisher Copyright:
© 2015, Springer International Publishing Switzerland.

Keywords

16S rRNA
Gram-negative
Lysobacter fragariae
Lysobacter rhizosphaerae
Ubiquinone-8

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10.1007/s10482-015-0439-x

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@article{b86d5a21802a46f6ac342173408f5a01,

title = "Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant",

abstract = "Two bacterial strains, designated THG-DN8.7T and THG-DN8.3T, were isolated from the rhizosphere of a strawberry plant in Gyeryong Mountain, South Korea. Cells of both isolates were observed to be Gram-negative, yellow-coloured and rod-shaped. Comparative 16S rRNA gene sequence analysis showed that strain THG-DN8.7T had highest sequence similarities to Lysobacter yangpyeongensis KACC 11407T (97.2 %), Lysobacter niabensis KACC 11587T (97.0 %) and Lysobacter oryzae KCTC 22249T (96.9 %), while strain THG-DN8.3T had closely similarity with L. niabensis KACC 11587T (98.1 %), L. oryzae KCTC 22249T (97.1 %) and L. yangpyeongensis KACC 11407T (96.1 %). DNA–DNA relatedness values between strains THG-DN8.7T and THG-DN8.3T and their closest phylogenetically neighbours were below 30.0 %, which indicates that strains THG-DN8.7T and THG-DN8.3T represent distinct species within the genus Lysobacter. Both strains were found to contain iso-C15:0, iso-C16:0 and iso-C17:1ω9c as predominant fatty acids and ubiquinone-8 as major isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strains THG-DN8.7T and THG-DN8.3T were determined to be 66.9 and 67.8 mol%, respectively. These data are consistent with the affiliation of the two new species represented by THG-DN8.7T and THG-DN8.3T to the genus Lysobacter. The names Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. are proposed for these species with the type strains THG-DN8.7T (=KCTC 42236T = JCM 30322T) and THG-DN8.3T (=KCTC 42237T = JCM 30321T), respectively.",

keywords = "16S rRNA, Gram-negative, Lysobacter fragariae, Lysobacter rhizosphaerae, Ubiquinone-8",

author = "Hina Singh and Juan Du and Ngo, {Hien T.T.} and Won, {Kyung Hwa} and Yang, {Jung Eun} and Kim, {Ki Young} and Yi, {Tae Hoo}",

note = "Publisher Copyright: {\textcopyright} 2015, Springer International Publishing Switzerland.",

year = "2015",

month = jun,

day = "22",

doi = "10.1007/s10482-015-0439-x",

language = "English",

volume = "107",

pages = "1437--1444",

journal = "Antonie van Leeuwenhoek",

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TY - JOUR

T1 - Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant

AU - Singh, Hina

AU - Du, Juan

AU - Ngo, Hien T.T.

AU - Won, Kyung Hwa

AU - Yang, Jung Eun

AU - Kim, Ki Young

AU - Yi, Tae Hoo

PY - 2015/6/22

Y1 - 2015/6/22

N2 - Two bacterial strains, designated THG-DN8.7T and THG-DN8.3T, were isolated from the rhizosphere of a strawberry plant in Gyeryong Mountain, South Korea. Cells of both isolates were observed to be Gram-negative, yellow-coloured and rod-shaped. Comparative 16S rRNA gene sequence analysis showed that strain THG-DN8.7T had highest sequence similarities to Lysobacter yangpyeongensis KACC 11407T (97.2 %), Lysobacter niabensis KACC 11587T (97.0 %) and Lysobacter oryzae KCTC 22249T (96.9 %), while strain THG-DN8.3T had closely similarity with L. niabensis KACC 11587T (98.1 %), L. oryzae KCTC 22249T (97.1 %) and L. yangpyeongensis KACC 11407T (96.1 %). DNA–DNA relatedness values between strains THG-DN8.7T and THG-DN8.3T and their closest phylogenetically neighbours were below 30.0 %, which indicates that strains THG-DN8.7T and THG-DN8.3T represent distinct species within the genus Lysobacter. Both strains were found to contain iso-C15:0, iso-C16:0 and iso-C17:1ω9c as predominant fatty acids and ubiquinone-8 as major isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strains THG-DN8.7T and THG-DN8.3T were determined to be 66.9 and 67.8 mol%, respectively. These data are consistent with the affiliation of the two new species represented by THG-DN8.7T and THG-DN8.3T to the genus Lysobacter. The names Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. are proposed for these species with the type strains THG-DN8.7T (=KCTC 42236T = JCM 30322T) and THG-DN8.3T (=KCTC 42237T = JCM 30321T), respectively.

AB - Two bacterial strains, designated THG-DN8.7T and THG-DN8.3T, were isolated from the rhizosphere of a strawberry plant in Gyeryong Mountain, South Korea. Cells of both isolates were observed to be Gram-negative, yellow-coloured and rod-shaped. Comparative 16S rRNA gene sequence analysis showed that strain THG-DN8.7T had highest sequence similarities to Lysobacter yangpyeongensis KACC 11407T (97.2 %), Lysobacter niabensis KACC 11587T (97.0 %) and Lysobacter oryzae KCTC 22249T (96.9 %), while strain THG-DN8.3T had closely similarity with L. niabensis KACC 11587T (98.1 %), L. oryzae KCTC 22249T (97.1 %) and L. yangpyeongensis KACC 11407T (96.1 %). DNA–DNA relatedness values between strains THG-DN8.7T and THG-DN8.3T and their closest phylogenetically neighbours were below 30.0 %, which indicates that strains THG-DN8.7T and THG-DN8.3T represent distinct species within the genus Lysobacter. Both strains were found to contain iso-C15:0, iso-C16:0 and iso-C17:1ω9c as predominant fatty acids and ubiquinone-8 as major isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strains THG-DN8.7T and THG-DN8.3T were determined to be 66.9 and 67.8 mol%, respectively. These data are consistent with the affiliation of the two new species represented by THG-DN8.7T and THG-DN8.3T to the genus Lysobacter. The names Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. are proposed for these species with the type strains THG-DN8.7T (=KCTC 42236T = JCM 30322T) and THG-DN8.3T (=KCTC 42237T = JCM 30321T), respectively.

KW - 16S rRNA

KW - Gram-negative

KW - Lysobacter fragariae

KW - Lysobacter rhizosphaerae

KW - Ubiquinone-8

UR - http://www.scopus.com/inward/record.url?scp=84937760110&partnerID=8YFLogxK

U2 - 10.1007/s10482-015-0439-x

DO - 10.1007/s10482-015-0439-x

M3 - Article

C2 - 25828767

AN - SCOPUS:84937760110

SN - 0003-6072

VL - 107

SP - 1437

EP - 1444

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

IS - 6

M1 - 8

ER -

Lysobacter fragariae sp. nov. and Lysobacter rhizosphaerae sp. nov. isolated from rhizosphere of strawberry plant

Abstract

Bibliographical note

Keywords

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