Lysobacter novalis sp. nov., isolated from fallow farmland soil

A novel bacterial strain, designated THG-PC7^T, was isolated from fallow farmland soil in Yongin, South Korea. Cells of strain THG-PC7^T were Gram-stain-negative, dark yellow, aerobic, rod-shaped and had gliding motility. Strain THG-PC7^T grew optimally at 25–35°C, at pH7 and in the absence of NaCl. Comparative 16S rRNA gene sequence analysis identified strain THG-PC7^T as belonging to the genus Lysobacter, exhibiting highest sequence similarity withLysobacter ximonensis KCTC 22336^T (98.7%) followed by Lysobacter niastensis KACC 11588^T(95.7%). In DNA–DNA hybridization tests, DNA relatedness between strain THG-PC7^T and its closest phylogenetic neighbour L. ximonensis was below 25%. The DNA G+C content of the novel isolate was determined to be 62.5mol%. Flexirubin-type pigments were found to be present. The major cellular fatty acids were determined to be iso-C_15:0, iso-C_16:0, anteiso-C_15:0 and iso-C_17:1ω9c. The major respiratory quinone was identified as ubiquonone-8 (Q8). The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospolipid. On the basis of results from DNA–DNA hybridization and the polyphasic data, strain THG-PC7^T represents a novel species of the genus Lysobacter, for which the name Lysobacter novalis sp. nov. is proposed. The type strain is THG-PC7^T(=KACC 18276^T=CCTCC AB 2014319^T).