Massilia arvi sp. nov., isolated from fallow-land soil previously cultivated with Brassica oleracea, and emended description of the genus Massilia

A novel bacterial strain, designated THG-RS2O^T, was isolated from fallow-land soil previously cultivated with Brassica oleracea in Yongin, South Korea. Cells were Gram-stain-negative, aerobic, non-motile rods, catalase- and oxidase-positive. Strain THG-RS2O^T grew optimally at 25-37 °C, at pH 7.0 and in the absence of NaCl. 16S rRNA gene sequence analysis demonstrated that strain THG-RS2O^T shows highest sequence similarity with Massilia kyonggiensis KACC 1 7471^T followed by Massilia aerilata KACC 12505^T, Massilia niastensis KACC 12599^T, Massilia tieshanensis KACC 14940T and Massilia haematophila KCTC 32001^T. Levels of DNA-DNA relatedness between strain THG-RS2O^T and the closest phylogenetic neighbours were below 55.0% and the DNA G + C content of strain THG-RS2O^T was 63.2 mol%. Major fatty acids were C₁₆:₀, cyclo-C₁₇:₀ and summed feature 3 (C₁₆:₁ω6c and/or C₁₆:₁ω7c). The major respiratory quinone was identified as ubiquonone-8 and predominant polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Characterization by 16S rRNA gene sequence analysis, DNA-DNA hybridization, ubiquinone, polar lipid, fatty acid composition, and physiological and biochemical parameters revealed that strain THG-RS2O^T represents a novel species of the genus Massilia. Hence, the present study describes a novel species for which the name Massilia arvi sp. nov. is proposed. The type strain is THG-RS2O^T (= KCTC 42609^T=CCTCC AB 2015115^T).