TY - JOUR
T1 - Matrix solid-phase dispersion extraction method for HPLC determination of flavonoids from buckwheat sprouts
AU - Mansur, Ahmad Rois
AU - Kim, Kwan Joong
AU - Kim, Dan Bi
AU - Yoo, Miyoung
AU - Jang, Hae Won
AU - Kim, Dae Ok
AU - Nam, Tae Gyu
N1 - Funding Information:
This research was supported by the Main Research Program (E0187200-03) of the Korea Food Research Institute funded by the Ministry of Science and ICT.
Funding Information:
This research was supported by the Main Research Program ( E0187200-03 ) of the Korea Food Research Institute funded by the Ministry of Science and ICT .
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/11
Y1 - 2020/11
N2 - Herein, we report the development of a matrix solid-phase dispersion (MSPD) extraction method for determining flavonoids (orientin, isoorientin, vitexin, isovitexin, quercetin-3-O-robinobioside, and rutin) from common buckwheat sprouts (CBS) and Tartary buckwheat sprouts (TBS) by high-performance liquid chromatography (HPLC-PDA). Key parameters, including sorbent type, sorbent-to-sample ratio, extraction solvent (type, concentration, and volume), and extraction time were optimized. The optimized MSPD extraction conditions include a C18-sorbent/sample ratio of 2:1 (w/w) [total mixture weight = 100 mg] and 10 min of static extraction with 5 mL of 80% ethanol. The optimized MSPD extraction method was satisfactorily validated for all target flavonoids from both CBS and TBS. The validated method was also more effective and efficient compared to ultrasound- and homogenate-assisted extraction techniques. The overall results show that the MSPD extraction method developed in this study is a simple, effective, and efficient technique for the HPLC analysis of flavonoids in CBS and TBS.
AB - Herein, we report the development of a matrix solid-phase dispersion (MSPD) extraction method for determining flavonoids (orientin, isoorientin, vitexin, isovitexin, quercetin-3-O-robinobioside, and rutin) from common buckwheat sprouts (CBS) and Tartary buckwheat sprouts (TBS) by high-performance liquid chromatography (HPLC-PDA). Key parameters, including sorbent type, sorbent-to-sample ratio, extraction solvent (type, concentration, and volume), and extraction time were optimized. The optimized MSPD extraction conditions include a C18-sorbent/sample ratio of 2:1 (w/w) [total mixture weight = 100 mg] and 10 min of static extraction with 5 mL of 80% ethanol. The optimized MSPD extraction method was satisfactorily validated for all target flavonoids from both CBS and TBS. The validated method was also more effective and efficient compared to ultrasound- and homogenate-assisted extraction techniques. The overall results show that the MSPD extraction method developed in this study is a simple, effective, and efficient technique for the HPLC analysis of flavonoids in CBS and TBS.
KW - Common buckwheat sprouts
KW - Flavonoids
KW - HPLC
KW - Matrix solid-phase dispersion
KW - Tartary buckwheat sprouts
UR - http://www.scopus.com/inward/record.url?scp=85090359734&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2020.110121
DO - 10.1016/j.lwt.2020.110121
M3 - Article
AN - SCOPUS:85090359734
VL - 133
JO - LWT - Food Science and Technology
JF - LWT - Food Science and Technology
SN - 0023-6438
M1 - 110121
ER -