Matrix solid-phase dispersion extraction method for HPLC determination of flavonoids from buckwheat sprouts

Ahmad Rois Mansur, Kwan Joong Kim, Dan Bi Kim, Miyoung Yoo, Hae Won Jang, Dae Ok Kim, Tae Gyu Nam

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Herein, we report the development of a matrix solid-phase dispersion (MSPD) extraction method for determining flavonoids (orientin, isoorientin, vitexin, isovitexin, quercetin-3-O-robinobioside, and rutin) from common buckwheat sprouts (CBS) and Tartary buckwheat sprouts (TBS) by high-performance liquid chromatography (HPLC-PDA). Key parameters, including sorbent type, sorbent-to-sample ratio, extraction solvent (type, concentration, and volume), and extraction time were optimized. The optimized MSPD extraction conditions include a C18-sorbent/sample ratio of 2:1 (w/w) [total mixture weight = 100 mg] and 10 min of static extraction with 5 mL of 80% ethanol. The optimized MSPD extraction method was satisfactorily validated for all target flavonoids from both CBS and TBS. The validated method was also more effective and efficient compared to ultrasound- and homogenate-assisted extraction techniques. The overall results show that the MSPD extraction method developed in this study is a simple, effective, and efficient technique for the HPLC analysis of flavonoids in CBS and TBS.

Original languageEnglish
Article number110121
JournalLWT - Food Science and Technology
Volume133
DOIs
Publication statusPublished - Nov 2020

Keywords

  • Common buckwheat sprouts
  • Flavonoids
  • HPLC
  • Matrix solid-phase dispersion
  • Tartary buckwheat sprouts

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