Measuring the activity of leucine-rich repeat kinase 2: A kinase involved in parkinson's disease

Byoung Dae Lee, Xiaojie Li, Ted M. Dawson, Valina L. Dawson

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)

Abstract

Mutations in the LRRK2 (Leucine-Rich Repeat Kinase 2) gene are the most common cause of autosomal dominant Parkinson's disease. LRRK2 has multiple functional domains including a kinase domain. The kinase activity of LRRK2 is implicated in the pathogenesis of Parkinson's disease. Developing an assay to understand the mechanisms of LRRK2 kinase activity is important for the development of pharmacologic and therapeutic applications. Here, we describe how to measure in vitro LRRK2 kinase activity and its inhibition.

Original languageEnglish
Title of host publicationKinase Inhibitors
Subtitle of host publicationMethods and Protocols
EditorsBernhard Kuster
Pages45-54
Number of pages10
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume795
ISSN (Print)1064-3745

Keywords

  • In vitro kinase assay
  • LRRK2
  • Parkinson's disease

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