@inproceedings{6b1b8d53aaf7485287d4af49e5c7c2f7,
title = "Microcantilever-based thrombin detection using a structure-switching aptamer as the receptor molecule",
abstract = "We report a sensitive nanomechanical detection of thrombin based on the structures witching DNA aptamer. The structural changes of the aptamer by thrombin binding were in situ monitored by measuring the resonance frequency of the piezoelectric microcantilevers. To evaluate the effect of structure-switching of duplex-to-complex by thrombin binding to the resonant frequency, the single stranded DNA aptamer and the guard DNA (g-DNA)-aptamer duplex were applied for the microcantilevers, and followed by thrombin binding at nanomolar concentration. We observed the thrombin binding-induced nanomechanical response from the two cases and could evaluate the g-DNA effect on the structure-switching signaling.",
keywords = "DNA aptamer, Guard DNA (g-DNA), Piezoelectric microcantilever, Thrombin",
author = "Lee, {Sang Myung} and Choi, {Jun Hwan} and Hwang, {Kyo Seon} and Kim, {Sang Kyung} and Kim, {Tae Song}",
year = "2009",
language = "English",
isbn = "9780979806421",
series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "1374--1376",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",
}