Mitigated toxicity of polystyrene nanoplastics in combination exposure with copper ions by transformation into copper (I) oxide: Inhibits the oxidative potential of nanoplastics

The combined impact of trace metals and polystyrene (PS) microplastics is extremely concerning for human health because PS microplastics can serve as a vehicle for other contaminants. Herein, we investigated the combined effect of copper ions (Cu²⁺) on the toxicity of PS nanoplastics in vivo and in vitro. The pristine PS (PPS) and ultraviolet irradiated oxidized PS (OPS) nanoplastics with 50 nm-size were conjugated with Cu²⁺ (13–27 mg/g) for 4 days to get four types of samples: PPS, OPS, PPS/Cu, and OPS/Cu. The comparative toxic potentials of test samples were evaluated using a mouse pharyngeal aspiration model and relevant human cell lines (A549 and differentiated THP-1 cells). The results showed an antagonistic effect in vivo and in vitro by the presence of Cu ions: PPS > PPS/Cu; OPS > OPS/Cu. Furthermore, the OPS produced significantly increased toxic potentials compared to the corresponding PPS: OPS > PPS; OPS/Cu > PPS/Cu. The antagonistic effect of Cu²⁺ on the toxicity of PS was due to the transformation of Cu²⁺ and balanced the surface charge of the nanoplastics, which inhibited the oxidative potential of corresponding nanoplastics. These antagonistic effects may provide a better understanding of the combined effects of metals on the intrinsic toxic potential of microplastics under natural conditions.