Monitoring biochemical changes during grape berry development in Portuguese cultivars by NMR spectroscopy

Kashif Ali; Federica Maltese; Ana Margarida Fortes; Maria Salomé Pais; Young Hae Choi; Robert Verpoorte

doi:10.1016/j.foodchem.2010.08.015

Monitoring biochemical changes during grape berry development in Portuguese cultivars by NMR spectroscopy

Kashif Ali, Federica Maltese, Ana Margarida Fortes, Maria Salomé Pais, Young Hae Choi, Robert Verpoorte

Research output: Contribution to journal › Article › peer-review

109 Citations (Scopus)

Abstract

¹H nuclear magnetic resonance (NMR) was applied for the metabolic profiling of grapes from three Portuguese cultivars including 'Trincadeira', 'Aragonês', and 'Touriga Nacional', at four developmental stages. Two kinds of extraction methods including deuterated NMR solvent extraction and solid phase extraction (SPE) were used for the metabolomic analysis and all the metabolites detected in ¹H NMR were elucidated by two-dimensional NMR techniques as well as the in-house NMR chemical shift database. Multivariate data analyses were also performed to identify overall metabolic differences. Trincadeira was found different from the other two cultivars, having low phenolic contents as compared to other cultivars. The initial stages showed comparatively high phenolics and organic acid contents like caftaric and malic acid while the later stages showed higher glucose and fructose levels. Veraison was found to be a metabolically critical stage of berry development. On the basis of these findings distribution of metabolites among different cultivars at different developmental stages is discussed.

Original language	English
Pages (from-to)	1760-1769
Number of pages	10
Journal	Food Chemistry
Volume	124
Issue number	4
DOIs	https://doi.org/10.1016/j.foodchem.2010.08.015
Publication status	Published - Feb 2011

Bibliographical note

Funding Information:
This work was supported by ERA-PG in Portugal under the project Genomic Research-Assisted breeding for Sustainable Production of Quality GRAPEs and WINE ( http://urgi.versailles.inra.fr/projects/GRASP/ ). The authors thank Higher Education Commission (HEC) of Pakistan for the financial support of Kashif Ali.

Keywords

Chemometrics
Developmental stages
Grapes
Nuclear magnetic resonance spectroscopy
Relative quantification

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10.1016/j.foodchem.2010.08.015

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title = "Monitoring biochemical changes during grape berry development in Portuguese cultivars by NMR spectroscopy",

abstract = "1H nuclear magnetic resonance (NMR) was applied for the metabolic profiling of grapes from three Portuguese cultivars including 'Trincadeira', 'Aragon{\^e}s', and 'Touriga Nacional', at four developmental stages. Two kinds of extraction methods including deuterated NMR solvent extraction and solid phase extraction (SPE) were used for the metabolomic analysis and all the metabolites detected in 1H NMR were elucidated by two-dimensional NMR techniques as well as the in-house NMR chemical shift database. Multivariate data analyses were also performed to identify overall metabolic differences. Trincadeira was found different from the other two cultivars, having low phenolic contents as compared to other cultivars. The initial stages showed comparatively high phenolics and organic acid contents like caftaric and malic acid while the later stages showed higher glucose and fructose levels. Veraison was found to be a metabolically critical stage of berry development. On the basis of these findings distribution of metabolites among different cultivars at different developmental stages is discussed.",

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author = "Kashif Ali and Federica Maltese and Fortes, {Ana Margarida} and Pais, {Maria Salom{\'e}} and Choi, {Young Hae} and Robert Verpoorte",

note = "Funding Information: This work was supported by ERA-PG in Portugal under the project Genomic Research-Assisted breeding for Sustainable Production of Quality GRAPEs and WINE ( http://urgi.versailles.inra.fr/projects/GRASP/ ). The authors thank Higher Education Commission (HEC) of Pakistan for the financial support of Kashif Ali. ",

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AU - Ali, Kashif

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AU - Fortes, Ana Margarida

AU - Pais, Maria Salomé

AU - Choi, Young Hae

AU - Verpoorte, Robert

N1 - Funding Information: This work was supported by ERA-PG in Portugal under the project Genomic Research-Assisted breeding for Sustainable Production of Quality GRAPEs and WINE ( http://urgi.versailles.inra.fr/projects/GRASP/ ). The authors thank Higher Education Commission (HEC) of Pakistan for the financial support of Kashif Ali.

PY - 2011/2

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N2 - 1H nuclear magnetic resonance (NMR) was applied for the metabolic profiling of grapes from three Portuguese cultivars including 'Trincadeira', 'Aragonês', and 'Touriga Nacional', at four developmental stages. Two kinds of extraction methods including deuterated NMR solvent extraction and solid phase extraction (SPE) were used for the metabolomic analysis and all the metabolites detected in 1H NMR were elucidated by two-dimensional NMR techniques as well as the in-house NMR chemical shift database. Multivariate data analyses were also performed to identify overall metabolic differences. Trincadeira was found different from the other two cultivars, having low phenolic contents as compared to other cultivars. The initial stages showed comparatively high phenolics and organic acid contents like caftaric and malic acid while the later stages showed higher glucose and fructose levels. Veraison was found to be a metabolically critical stage of berry development. On the basis of these findings distribution of metabolites among different cultivars at different developmental stages is discussed.

AB - 1H nuclear magnetic resonance (NMR) was applied for the metabolic profiling of grapes from three Portuguese cultivars including 'Trincadeira', 'Aragonês', and 'Touriga Nacional', at four developmental stages. Two kinds of extraction methods including deuterated NMR solvent extraction and solid phase extraction (SPE) were used for the metabolomic analysis and all the metabolites detected in 1H NMR were elucidated by two-dimensional NMR techniques as well as the in-house NMR chemical shift database. Multivariate data analyses were also performed to identify overall metabolic differences. Trincadeira was found different from the other two cultivars, having low phenolic contents as compared to other cultivars. The initial stages showed comparatively high phenolics and organic acid contents like caftaric and malic acid while the later stages showed higher glucose and fructose levels. Veraison was found to be a metabolically critical stage of berry development. On the basis of these findings distribution of metabolites among different cultivars at different developmental stages is discussed.

KW - Chemometrics

KW - Developmental stages

KW - Grapes

KW - Nuclear magnetic resonance spectroscopy

KW - Relative quantification

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SN - 0308-8146

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SP - 1760

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JO - Food Chemistry

JF - Food Chemistry

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ER -

Monitoring biochemical changes during grape berry development in Portuguese cultivars by NMR spectroscopy

Abstract

Bibliographical note

Keywords

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