Mucilaginibacter ginsenosidivorax sp. nov., with ginsenoside converting activity isolated from sediment

Jin Kwang Kim; Tae Eun Choi; Qing Mei Liu; Hye Yoon Park; Tae Hoo Yi; Min Ho Yoon; Sun Chang Kim; Wan Taek Im

doi:10.1007/s12275-013-2653-4

Mucilaginibacter ginsenosidivorax sp. nov., with ginsenoside converting activity isolated from sediment

Jin Kwang Kim
, Tae Eun Choi
, Qing Mei Liu
, Hye Yoon Park
, Tae Hoo Yi
, Min Ho Yoon
, Sun Chang Kim
, Wan Taek Im

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated KHI28^T was isolated from sediment in Gapcheon (river) and its taxonomic position was investigated using a polyphasic approach. Strain KHI28^T grew at 10-42°C and at pH 5.5-8.5 on R2A and nutrient agar without additional NaCl as a supplement. Strain KHI28^T possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb₁ and Re (ones of the dominant active components of ginseng) to C-K and Rg₂, respectively. On the basis of 16S rRNA gene sequence similarity, strain KHI28^T was shown to belong to the family Sphingobacteriaceae and to be related to Mucilaginibacter dorajii DR-f4^T (97.9% sequence similarity), M. polysacchareus DRP28^T (97.3%), and M. lappiensis ANJLI2^T (97.2%). The G+C content of the genomic DNA was 45.8%. The predominant respiratory quinone was MK-7 and the major fatty acids were summed feature 3 (comprising C_16:1 ω6c and/or C_16:1 ω7c), iso-C_15:0 and C_16:0. DNA and chemotaxonomic data supported the affiliation of strain KHI28^T to the genus Mucilaginibacter. Strain KHI28^T could be differentiated genotypically and phenotypically from the recognized species of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter ginsenosidivorax sp. nov. is proposed, with the type strain KHI28^T (=KACC 14955^T =LMG 25804^T).

Original language	English
Pages (from-to)	394-399
Number of pages	6
Journal	Journal of Microbiology
Volume	51
Issue number	3
DOIs	https://doi.org/10.1007/s12275-013-2653-4
Publication status	Published - Jun 2013

Bibliographical note

Funding Information:
This work was supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031967) and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Korea.

Keywords

16S rRNA gene
Mucilaginibacter ginsenosidivorax
ginsenoside
polyphasic taxonomy

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10.1007/s12275-013-2653-4

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@article{53f34c3020444f5fbaf90a79f93e08ab,

title = "Mucilaginibacter ginsenosidivorax sp. nov., with ginsenoside converting activity isolated from sediment",

abstract = "A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated KHI28T was isolated from sediment in Gapcheon (river) and its taxonomic position was investigated using a polyphasic approach. Strain KHI28T grew at 10-42°C and at pH 5.5-8.5 on R2A and nutrient agar without additional NaCl as a supplement. Strain KHI28T possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1 and Re (ones of the dominant active components of ginseng) to C-K and Rg2, respectively. On the basis of 16S rRNA gene sequence similarity, strain KHI28T was shown to belong to the family Sphingobacteriaceae and to be related to Mucilaginibacter dorajii DR-f4T (97.9\% sequence similarity), M. polysacchareus DRP28T (97.3\%), and M. lappiensis ANJLI2T (97.2\%). The G+C content of the genomic DNA was 45.8\%. The predominant respiratory quinone was MK-7 and the major fatty acids were summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c), iso-C15:0 and C16:0. DNA and chemotaxonomic data supported the affiliation of strain KHI28T to the genus Mucilaginibacter. Strain KHI28T could be differentiated genotypically and phenotypically from the recognized species of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter ginsenosidivorax sp. nov. is proposed, with the type strain KHI28T (=KACC 14955T =LMG 25804T).",

keywords = "16S rRNA gene, Mucilaginibacter ginsenosidivorax, ginsenoside, polyphasic taxonomy",

author = "Kim, \{Jin Kwang\} and Choi, \{Tae Eun\} and Liu, \{Qing Mei\} and Park, \{Hye Yoon\} and Yi, \{Tae Hoo\} and Yoon, \{Min Ho\} and Kim, \{Sun Chang\} and Im, \{Wan Taek\}",

note = "Funding Information: This work was supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031967) and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Korea.",

year = "2013",

month = jun,

doi = "10.1007/s12275-013-2653-4",

language = "English",

volume = "51",

pages = "394--399",

journal = "Journal of Microbiology",

issn = "1225-8873",

publisher = "Microbiological Society of Korea",

number = "3",

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TY - JOUR

T1 - Mucilaginibacter ginsenosidivorax sp. nov., with ginsenoside converting activity isolated from sediment

AU - Kim, Jin Kwang

AU - Choi, Tae Eun

AU - Liu, Qing Mei

AU - Park, Hye Yoon

AU - Yi, Tae Hoo

AU - Yoon, Min Ho

AU - Kim, Sun Chang

AU - Im, Wan Taek

N1 - Funding Information: This work was supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031967) and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Korea.

PY - 2013/6

Y1 - 2013/6

N2 - A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated KHI28T was isolated from sediment in Gapcheon (river) and its taxonomic position was investigated using a polyphasic approach. Strain KHI28T grew at 10-42°C and at pH 5.5-8.5 on R2A and nutrient agar without additional NaCl as a supplement. Strain KHI28T possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1 and Re (ones of the dominant active components of ginseng) to C-K and Rg2, respectively. On the basis of 16S rRNA gene sequence similarity, strain KHI28T was shown to belong to the family Sphingobacteriaceae and to be related to Mucilaginibacter dorajii DR-f4T (97.9% sequence similarity), M. polysacchareus DRP28T (97.3%), and M. lappiensis ANJLI2T (97.2%). The G+C content of the genomic DNA was 45.8%. The predominant respiratory quinone was MK-7 and the major fatty acids were summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c), iso-C15:0 and C16:0. DNA and chemotaxonomic data supported the affiliation of strain KHI28T to the genus Mucilaginibacter. Strain KHI28T could be differentiated genotypically and phenotypically from the recognized species of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter ginsenosidivorax sp. nov. is proposed, with the type strain KHI28T (=KACC 14955T =LMG 25804T).

AB - A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated KHI28T was isolated from sediment in Gapcheon (river) and its taxonomic position was investigated using a polyphasic approach. Strain KHI28T grew at 10-42°C and at pH 5.5-8.5 on R2A and nutrient agar without additional NaCl as a supplement. Strain KHI28T possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1 and Re (ones of the dominant active components of ginseng) to C-K and Rg2, respectively. On the basis of 16S rRNA gene sequence similarity, strain KHI28T was shown to belong to the family Sphingobacteriaceae and to be related to Mucilaginibacter dorajii DR-f4T (97.9% sequence similarity), M. polysacchareus DRP28T (97.3%), and M. lappiensis ANJLI2T (97.2%). The G+C content of the genomic DNA was 45.8%. The predominant respiratory quinone was MK-7 and the major fatty acids were summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c), iso-C15:0 and C16:0. DNA and chemotaxonomic data supported the affiliation of strain KHI28T to the genus Mucilaginibacter. Strain KHI28T could be differentiated genotypically and phenotypically from the recognized species of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter ginsenosidivorax sp. nov. is proposed, with the type strain KHI28T (=KACC 14955T =LMG 25804T).

KW - 16S rRNA gene

KW - Mucilaginibacter ginsenosidivorax

KW - ginsenoside

KW - polyphasic taxonomy

UR - https://www.scopus.com/pages/publications/84879667087

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DO - 10.1007/s12275-013-2653-4

M3 - Article

C2 - 23812820

AN - SCOPUS:84879667087

SN - 1225-8873

VL - 51

SP - 394

EP - 399

JO - Journal of Microbiology

JF - Journal of Microbiology

IS - 3

ER -

Mucilaginibacter ginsenosidivorax sp. nov., with ginsenoside converting activity isolated from sediment

Abstract

Bibliographical note

Keywords

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