Multiplex SNP analysis microdevice using allele-specific polymerase chain reaction-microarray

Jong Young Choi, Yong Tae Kim, Ju Young Byun, Jinwoo Ahn, Dae Gab Gweon, Min Gon Kim, Tae Seok Seo

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this study, we developed an allele-specific polymerase chain reaction-microarray (AS PCR-microarray) chip to perform the allele-specific gene amplification and microarray-based detection on a single wafer to identify Korean indigenous beef cattle, called Hanwoo. For allele-specific discrimination and amplification, Hanwoo-specific ten homozygous single nucleotide polymorphism (SNP) sites were chosen and analyzed by AS PCR on a chip, and the resultant amplicons were verified by the downstream microarray assay. We could identify Hanwoo with 99.3% reliability by detecting the fluorescence signals of the microarray less than three among eleven spots.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages932-934
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Allele-specific polymerase chain reaction
  • Integrated microdevice
  • Microarray
  • Snp typing

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