Mussel-inspired poly(L-DOPA)-templated mineralization for calcium phosphate-assembled intracellular nanocarriers

Hye Young Nam; Kyung Hyun Min; Da Eun Kim; Jeong Ryul Choi; Hong Jae Lee; Sang Cheon Lee

doi:10.1016/j.colsurfb.2017.05.077

Mussel-inspired poly(L-DOPA)-templated mineralization for calcium phosphate-assembled intracellular nanocarriers

Hye Young Nam, Kyung Hyun Min, Da Eun Kim, Jeong Ryul Choi, Hong Jae Lee, Sang Cheon Lee

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

We developed a calcium phosphate (CaP)-assembled polymer nanocarrier for intracellular doxorubicin (DOX) delivery based on a mussel-inspired mineralization approach. A DOX-loaded core-shell polymer nanoparticle (DOX-NP) consisting of a poly(3,4-dihydroxy-L-phenylalanine) (PDOPA) core and a poly (ethylene glycol) (PEG) shell was utilized as a nanotemplate for CaP mineralization. The mean hydrodynamic diameter of the DOX-loaded CaP-mineralized polymer nanoparticles (DOX-CaP-NPs) was 154.3 nm. Energy-dispersive X-ray spectroscopy confirmed that the DOX-CaP-NPs contained substantial amounts of Ca and P, elements found only in the CaP mineral. The loading efficiency and content of DOX, estimated by fluorescence spectroscopy, were 54.0% and 10.8 wt%, respectively. The CaP deposited in the PDOPA core domain enabled the DOX-CaP-NPs to maintain a robust structure and effectively inhibit DOX release at extracellular pH, whereas at endosomal pH, the CaP core dissolved to trigger a facilitated DOX release. The DOX-CaP-NPs may serve as robust nanocarriers with a high delivery efficacy for cancer chemotherapy.

Original language	English
Pages (from-to)	215-222
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	157
DOIs	https://doi.org/10.1016/j.colsurfb.2017.05.077
Publication status	Published - 1 Sept 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

Calcium phosphate
DOPA
Intracellular delivery
Mineralization
Mussel

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.colsurfb.2017.05.077

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title = "Mussel-inspired poly(L-DOPA)-templated mineralization for calcium phosphate-assembled intracellular nanocarriers",

abstract = "We developed a calcium phosphate (CaP)-assembled polymer nanocarrier for intracellular doxorubicin (DOX) delivery based on a mussel-inspired mineralization approach. A DOX-loaded core-shell polymer nanoparticle (DOX-NP) consisting of a poly(3,4-dihydroxy-L-phenylalanine) (PDOPA) core and a poly (ethylene glycol) (PEG) shell was utilized as a nanotemplate for CaP mineralization. The mean hydrodynamic diameter of the DOX-loaded CaP-mineralized polymer nanoparticles (DOX-CaP-NPs) was 154.3 nm. Energy-dispersive X-ray spectroscopy confirmed that the DOX-CaP-NPs contained substantial amounts of Ca and P, elements found only in the CaP mineral. The loading efficiency and content of DOX, estimated by fluorescence spectroscopy, were 54.0% and 10.8 wt%, respectively. The CaP deposited in the PDOPA core domain enabled the DOX-CaP-NPs to maintain a robust structure and effectively inhibit DOX release at extracellular pH, whereas at endosomal pH, the CaP core dissolved to trigger a facilitated DOX release. The DOX-CaP-NPs may serve as robust nanocarriers with a high delivery efficacy for cancer chemotherapy.",

keywords = "Calcium phosphate, DOPA, Intracellular delivery, Mineralization, Mussel",

author = "Nam, {Hye Young} and Min, {Kyung Hyun} and Kim, {Da Eun} and Choi, {Jeong Ryul} and Lee, {Hong Jae} and Lee, {Sang Cheon}",

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T1 - Mussel-inspired poly(L-DOPA)-templated mineralization for calcium phosphate-assembled intracellular nanocarriers

AU - Nam, Hye Young

AU - Min, Kyung Hyun

AU - Kim, Da Eun

AU - Choi, Jeong Ryul

AU - Lee, Hong Jae

AU - Lee, Sang Cheon

PY - 2017/9/1

Y1 - 2017/9/1

N2 - We developed a calcium phosphate (CaP)-assembled polymer nanocarrier for intracellular doxorubicin (DOX) delivery based on a mussel-inspired mineralization approach. A DOX-loaded core-shell polymer nanoparticle (DOX-NP) consisting of a poly(3,4-dihydroxy-L-phenylalanine) (PDOPA) core and a poly (ethylene glycol) (PEG) shell was utilized as a nanotemplate for CaP mineralization. The mean hydrodynamic diameter of the DOX-loaded CaP-mineralized polymer nanoparticles (DOX-CaP-NPs) was 154.3 nm. Energy-dispersive X-ray spectroscopy confirmed that the DOX-CaP-NPs contained substantial amounts of Ca and P, elements found only in the CaP mineral. The loading efficiency and content of DOX, estimated by fluorescence spectroscopy, were 54.0% and 10.8 wt%, respectively. The CaP deposited in the PDOPA core domain enabled the DOX-CaP-NPs to maintain a robust structure and effectively inhibit DOX release at extracellular pH, whereas at endosomal pH, the CaP core dissolved to trigger a facilitated DOX release. The DOX-CaP-NPs may serve as robust nanocarriers with a high delivery efficacy for cancer chemotherapy.

AB - We developed a calcium phosphate (CaP)-assembled polymer nanocarrier for intracellular doxorubicin (DOX) delivery based on a mussel-inspired mineralization approach. A DOX-loaded core-shell polymer nanoparticle (DOX-NP) consisting of a poly(3,4-dihydroxy-L-phenylalanine) (PDOPA) core and a poly (ethylene glycol) (PEG) shell was utilized as a nanotemplate for CaP mineralization. The mean hydrodynamic diameter of the DOX-loaded CaP-mineralized polymer nanoparticles (DOX-CaP-NPs) was 154.3 nm. Energy-dispersive X-ray spectroscopy confirmed that the DOX-CaP-NPs contained substantial amounts of Ca and P, elements found only in the CaP mineral. The loading efficiency and content of DOX, estimated by fluorescence spectroscopy, were 54.0% and 10.8 wt%, respectively. The CaP deposited in the PDOPA core domain enabled the DOX-CaP-NPs to maintain a robust structure and effectively inhibit DOX release at extracellular pH, whereas at endosomal pH, the CaP core dissolved to trigger a facilitated DOX release. The DOX-CaP-NPs may serve as robust nanocarriers with a high delivery efficacy for cancer chemotherapy.

KW - Calcium phosphate

KW - DOPA

KW - Intracellular delivery

KW - Mineralization

KW - Mussel

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Mussel-inspired poly(L-DOPA)-templated mineralization for calcium phosphate-assembled intracellular nanocarriers

Abstract

Bibliographical note

Keywords

UN SDGs

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